Open Access

The promising role of epigenetic mediators and microRNAs in the early diagnosis of cholangiocarcinoma (Review)

  • Authors:
    • Vikrant Rai
    • Chandra S. Boosani
    • Devendra K. Agrawal
  • View Affiliations

  • Published online on: August 9, 2019     https://doi.org/10.3892/wasj.2019.18
  • Pages: 165-176
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Abstract

Cholangiocarcinoma (CC) is a highly lethal malignant tumor which arises from the biliary tract epithelium and is notoriously difficult to diagnose. Common risk factors for CC are primary sclerosing cholangitis, liver fluke infestation and hepatolithiasis. Although CCs are relatively uncommon tumors, the worldwide rising incidences and mortality rate for intrahepatic CC (ICC) renders it a disease of interest for research. CCs are usually fatal due to the typically late clinical presentation and the lack of effective non‑surgical therapeutic modalities. The overall survival rate, including following tumor resection, is poor with <5% of patients surviving 5 years and this rate has not significantly improved over the past 30 years. Thus, there is a need to diagnose CC at an early stage, and advances in immunohistochemistry, molecular genetics, pharmacogenomics and personalized medicine may aid in the study of the pathological basis of CC at the gene and protein level. Understanding the genetic and proteomic alterations in CC would not only increase the therapeutic efficacy, but would also provide a better treatment strategy. Epigenetic alterations that induce gene expression in cancers have been well established. Among the epigenetic mechanisms, targeting DNA hypermethylation and histone deacetylation with DNA methyltransferase (DNMT) and histone deacetylase (HDAC) inhibitors has been reported in a number of cancer types. In CC, targeting the epigenetic pathways appears to be a promising approach for treatment. This review aims to provide a comprehensive overview of the putative role of epigenetic alterations and proteomic alterations in CC. Furthermore, the role of these alterations in early diagnosis, as prognostic markers, and therapeutics for better treatment strategies will be highlighted.

1. Introduction

Cholangiocarcinoma (CC), arising from the biliary tract, is a rare malignant tumor accounting for almost 3% of all gastrointestinal cancers (1). On the basis of their location, two types of CCs have been characterized, namely intrahepatic CC (ICC) or extrahepatic CC (ECC) with clinical, pathological, epidemiological and molecular differences between them (2). The third one, which is considered as a sub-type of intrahepatic CC has also been described. These hilar cancers (Klatskin tumors) represents the most frequent category comprising 55-60% of CCs. Non-hilar ECC comprises 20-30% and ICC 10% of the total CC cases. More than 90% of CCs are adenocarcinomas (3). The common risk factors for CCs are primary sclerosing cholangitis (PSC), liver fluke infestation, congenital abnormalities, chronic hepatitis B virus infection, chronic hepatitis C virus infection, and hepatolithiasis (4). Due to the typically clinically late diagnosis and unresectable disease at presentation and the lack of effective non-surgical therapeutic modalities, CCs are usually fatal and patients usually succumb to the disease within 12 months. Cancer cachexia, liver failure, recurrent sepsis secondary to biliary obstruction and the subsequent rapid decline in performance status mainly contribute to the high mortality rate associated with this type of cancer. The overall survival rate, including in patients who have undergone tumor resection, is poor, with <5% of patients surviving 5 years. The poor survival rate has not improved significantly over the past 30 years (5). Although CC is a relatively rare tumor, the rising interest in this disease is due to the increasing incidence and mortality rates for ICC worldwide and the cause behind these remains unclear (4-9). Early lymph node metastasis and perineural invasion account for the poor outcome of patients with CC (10). Surgical liver resection with clear margins has been considered as the standard treatment for resectable CC; however, the survival rates are poor. Liver transplant has yielded some recent excellent results in highly selected patients with hilar CC; however, the major limitation is the shortage of cadaveric donor organs (11).

2. Diagnostic snags

Despite the development of the diagnostic and therapeutic modalities for various diseases and cancers, CC remains difficult to diagnose and continues to be a highly lethal disease with an extremely poor response to conventional anticancer therapies and a poor survival rate (4,6). The poor prognosis and survival rate associated with CC is mainly due to the lack of early diagnosis. Although molecular markers, including CA 19-9, carcinoembryonic antigen (CEA), CA-125, platelet-derived growth factor and basic fibroblast growth factor are being used for diagnosis, these markers lack the sensitivity and specificity in early disease. Furthermore, CA19-9 has good sensitivity and specificity for CC, but not for CC unassociated with primary sclerosing cholangitis (12). Thus, there is a need for the development of more effective and reliable markers for the early detection of CC. The study of genetic and epigenetic alterations mediating the molecular alterations and the malignant transformation of biliary cells occurring in CC may foster novel diagnostic, prognostic and therapeutic approaches (13). Developing interest in the molecular medicine and molecular genetics in the context of personalized, preventive, predictive and participatory medicine to provide better medical care in order to decrease the incidence and prevalence of the disease, as well as the study of the epigenetic alterations for the identification of genes involved in the tumorigenesis may prove to be beneficial (14). Since epigenetic alterations in gene expression are associated with CC, genes that are differentially methylated in CC may be useful in providing valuable information on potential markers for the detection of early-stage curable disease, markers prognostic of response to specific treatments and overall prognosis and novel targets for the design of rational therapies (4,6).

3. Mechanism of tumorigenesis

The accumulation of various defective cancerous or mutated genes results in the activation of multistep processes to induce tumorigenesis, resulting in CC, which is characterized by the activation of growth-promoting genes and the silencing of tumor growth suppressor genes mediating the uncontrolled proliferation by altering the tissue homeostasis favorable for increasing the cell proliferation rate, decreasing cell death rate, and creating a growth-promoting environment (15). The main alterations in cancer gene functions in cell physiology are self-sufficiency in growth signals, insensitivity to growth-inhibitory signals, the evasion of apoptosis, limitless replicative potential, sustained angiogenesis and tissue invasion and metastasis (16). These changes may be due to genetic alterations in oncogenes or due to epigenetic alterations. Two major mechanisms, DNA methylation which adds methyl groups at CpG sites, to convert cytosine to 5-methylcytosine, and the post-translational histone modifications comprises the common epigenetic mechanisms in cancer (4,6).

Epigenetics is an evolving area of research which can provide deeper insight, and improved diagnostics and theranostics for a number of human diseases. Epigenetic modifications include both heritable and non-heritable changes that regulate gene expression without altering the DNA sequence. Three major epigenetic mechanisms have been identified which are mediated through DNA methylation, histone modifications and non-coding RNAs. In DNA methylation, the cytosine residue at the CG sequence in DNA is specifically methylated, involving DNA methyltransferases (DNMTs), which results in the formation of 5-methycytosine. Subsequently, the methylated DNA fails to become transcribed and this results in gene silencing (4,6). More than 11 different types of post-translational modifications on histone proteins have been described. Based on the type of modification on specific residues of histone proteins, gene expression becomes affected or induced, suggesting that histone modifications epigenetically regulate gene expression. Non-coding RNAs, which include microRNAs (miRNAs or miRs), siRNAs and long non-coding RNAs (lncRNAs) have also been identified to epigenetically affect gene expression. With the exception of lncRNAs, these RNA species bind to the complementary sequence on messenger RNA (mRNA) transcripts and thereby prevent protein translation. In a number of cancer types and in different human diseases, multiple different epigenetic mechanisms have been identified as key regulators which can induce and enhance the progression of disease. Therefore, targeting epigenetic mediators is considered to be very promising in drug development for the treatment of various human diseases (4,5,13,17,18).

4. Epigenetic alterations in cholangiocarcinoma

DNA methylation

Studies have suggested that a number of genetic and epigenetic alterations occur during the neoplastic transformation of biliary epithelial cells that lead to the malignant progression of CC (13,19,20). DNA methylation is the most well-studied epigenetic mechanism in CC. In CC tumorigenesis, the promoter regions of tumor suppressor genes are heavily methylated (promoter hypermethylation), leading to gene silencing. Genomic DNA is less methylated (global hypomethylation), resulting in increased genomic instability and the reactivation of transposon elements (4,21,22). In CC, the promoter hypermethylation of genes involved in the cell cycle, cell adhesion, DNA repair, apoptosis and carcinogen/drug metabolism have been reported (4,6). The most common cancer-related genes studied thus far in relation to CC are K-ras, p53, p14 alternate reading frame gene (p14ARF), p16INK4a, SFRP1, SFRP2 and β-catenin (4,6,22) (Table I). The majority of K-ras gene mutations occur in codon 12. Genetic alterations, such as point mutations of K-ras and p53 have been frequently found in a subset of CC cases (24-27); however, the mutation or deletion of the cell cycle regulators, p14ARF and p16INK4a, are not frequent (28,29). Although β-catenin overexpression is frequently encountered in CC, mutations in the β-catenin gene have not been identified in ICC to date, at least to the best of our knowledge (30). These results suggest the crucial role of DNA methylation in the tumorigenesis of CC and the potential of studying these epigenetic alterations in order to identify and develop improved and more effective therapeutic modalities in the future (22) (Table I).

Table I

DNA methylation in the genomic sequences of specific genes that are associated with the pathogenesis of cholangiocarcinoma.

Table I

DNA methylation in the genomic sequences of specific genes that are associated with the pathogenesis of cholangiocarcinoma.

Gene (location)Function (Refs.)Epigenetic modification/effect (Refs.)Outcome (Refs.)
p16INK4A or CDKN2A (9p21)Tumor suppressor gene Regulates cell proliferation and oncogenesis (31)Promoter region hypermethylation of the p16INK4A results in gene inactivationMore frequent in ECC cases (38)
  Common event in PSC-associated CC (28)More commonly observed in tumors with vascular invasion (38)
  MF=17.7 to 83% (20,32-35), 25% PSC-CC, 28.3%, liver fluke CC, and 100% hepatolithiasis CC (28,36,37)Poor clinical outcome (20)
RASSF1A (3p21.3)Tumor suppressor gene induces cell cycle arrest by inhibiting the accumulation of cyclin D1Hypermethylation of its CpG island promoter region results in inactivation MF=27-69% (32,34,39)Promoter methylation is more common in ECC than ICC (32)
hMLH1 (3p21.3)DNA mismatch repair genePromoter methylation/hypermethylation of the hMLH1 geneMethylation frequencies vary in sporadic CC, biliary papillary, neoplasms, and liver fluke-related CC
  MF=8.1 and 25% sporadic CC (32,34), 0% biliary papillary CC (40), 44.6% Fluke-related CC (41)Associated with poorly differentiated subtype of CC with vascular invasion (41)
FHIT (3p14.2)Tumor suppressor genePromoter hypermethylation of the FHIT gene results in epigenetic silencing of the FHIT promoter region MF=42% (42)Development of intrahepatic CCs
14-3-3Tumor suppressor gene. Regulates cell cycle and cell deathCpG island hypermethylation cause inactivation of gene MF=59.5% CC (20)Reported in ICC (20)
TMS1/ASC (16p11.2)Tumor suppressor geneAberrant methylation of the TMS1/ASC cause inactivation of gene MF=36.1% CC (43)Associated with CC (43)
APC (5q21–q22)Tumor suppressor gene Controls cell division, cell-cell interactions and cell migration and invasion, and conservation of chromosomal number during cell divisionAPC gene hypermethylation MF=26.6 to 46% CC (20,32)Worse clinical outcome in CC (20,32)
DAPK (9q34.1)Tumor suppressor gene Positive mediator of interferon-γ (IFN-γ)-induced programmed cell deathDAPK gene hypermethylation MF=3 to 21.4% CC (32,34)Associated with poorly differentiated CCs and with a poor prognosis (32,34)
Epithelial (E) cadherin gene (16q22.1)Tumor suppressor geneHypermethylation of the promoter region of E gene Results in loss of function and contribute to progression of cancer by increasing proliferation, invasion and metastasis MF=21.5 to 43% CC (20,32,34,43,44)Development of intrahepatic CC
RAR-β (or HAP, RRB2 and NR1B2) (3p24)Mediates cellular signaling in embryonic morphogenesis, cell growth and differentiation by regulating gene expressionGene silencing by promoter region hypermethylation Results in increased tumorigenesis MF=14% of CCs (32)Increased tumorigenesis in CC
p73 gene (1p36.3)Tumor suppressor gene and related to the p53 genePromoter region hypermethylation increased tumorigenesis MF=36% CC (32)Increased tumorigenesis in CC
MGMT gene (10q26)Responsible for repairing alkylation. DNA damage inhibits estrogen receptor-mediated cell proliferationMethylation of discrete regions of the MGMT CpG island, results in heterochromatinization of the MGMT transcription start site and silencing of the gene MF=33% (32) and 49% CC (44)Increased frequency of GC to AT transitions in oncogenes and tumor suppressor genes and a poor prognosis (44)
GSTP gene (1q43)Regulate drug and xenobiotic. metabolismPromoter region hypermethylation (32) MF=<15% CC (20)Hypermethylation more frequent in ICC than in ECC (32)
CHFR gene (12q24.33)Tumor suppressor gene Delays the entry into the metaphaseGene silencing by promoter hypermethylation MF=16.2% in biliary tract carcinomas (34)Increased tumorigenesis in CC
RUNX3 gene (Ip36)Tumor suppressor gene Regulate proliferation of the biliary tract epitheliumMethylation of RUNX3 results in gene silencing MF=56.8% in biliary tract cancer (34)Associated with poorer survival (34)
TIMP3 gene (22q12.3)Plays a role in the induction of apoptosisCpG island methylation of TIMP3 gene MF=8.9 and 9% CC (20,32)Associated with worse survival (20)
SEMA3B (3p21.3)Tumor suppressor gene by inducing apoptosis. Plays a critical role in the guidance of growth cones during neuronal developmentMethylation of SEMA3B gene MF=100% CC (45)Increased tumorigenesis in CC
BLU gene (3p21.3)Tumor suppressor geneGene methylation MF=20% CC (45)Increased tumorigenesis in CC
THBS1 gene (15q15)Mediates cell-to-cell and cell-to-matrix interactions and play roles in platelet aggregation, angiogenesis and tumorigenesisHypermethylation in the promoter region of THBS1 gene MF=11% CC (20,45)Increased tumorigenesis in CC
p14ARF (9p21)Encoded by the β transcript of CDKN2A (p16/CDKN2A)Methylation of p14ARF MF=38 and 25% (32,35); 40.2% liver fluke CC (37)Increased tumorigenesis in CC
p15INK4b or p15 (9p21)Effecter of TGF-β-mediated cell cycle arrestPromoter hypermethylation of p15 gene, MF=50% of CC (32), 48.9% liver fluke CC (37)Increased tumorigenesis in CC
COX-2/PTGS2 (1q25.2–q25.3)Acts both as a dioxygenase and as a peroxidaseMethylation of COX-2 gene, MF=5.1% of CC (20)Increased tumorigenesis in CC
OPCMLTumor suppressor geneHypermethylation of OPCML, MF=72.5% Fluke CC (46)Increased tumorigenesis in CC
RIZ1Tumor suppressor geneMethylation of RIZ1 Results in chromatin compaction and gene silencing MF=38% liver fluke CC (47)Increased proliferation and migration of CC cell line (48)

[i] CC, cholangiocarcinoma; ECC, extrahepatic cholangiocarcinoma; ICC, intrahepatic cholangiocarcinoma; PSC, primary sclerosing cholangitis; MF, methylation frequency; O6-methylguanine-DNA methyltransferase; CDKN2A, cyclin-dependent kinase inhibitor 2A.

Methylated CpG islands in tumor genes termed methylated in tumor gene (MINT) are associated with carcinogenesis of the biliary tract epithelium and other epithelial cancers. The MINT loci associated with CC may be CpG island methylator phenotype (CIMP)-positive or -negative, depending on the histological type of CC (20). The methylation of various genes presented in Table I is associated with a poor survival and increased tumorigenesis; however, the methylation of DcR1, the decoy receptor, is associated with a significantly longer overall survival. This suggests that the identification of specific epigenetic alterations may serve as a prognostic marker in CC. Furthermore, the positivity of epigenetic alterations in the less differentiated CC, but not in normal adjacent tissue, suggests the potential role of epigenetic biomarkers for prognosis and diagnosis (46). Furthermore, intraepithelial biliary neoplasms (IEBNs), the mucosal extension of carcinoma and preinvasive neoplastic lesions in the bile ducts around CC have been found to be associated with nodular-sclerosing CC (NSCC), a common CC of the intrahepatic large, perihilar and distal bile ducts. Immunohistochemical analysis with S100P, vimentin, S100A4, E-cadherin, MUC1, MUC2, MUC5AC, MUC6, CDX2, CK7, CK20, CDX2, CD10, p53 and Ki67 in NSCC has revealed a pre-invasive and cancerous lesion zone (49). Since, epigenetic alterations are found in less differentiated CC, but not in normal adjacent tissue, studying the epigenetic and proteomic alterations in pre-invasive compared to cancerous lesions, may prove to be beneficial for the development of more effective treatment strategies.

Histone modifications

Histones, complex with genomic DNA to form nucleosomes, which consist of two turns of DNA wrapped around a histone octamer composed of two subunits of each histone, H2A, H2B, H3 and H4, with H1 as the linker histone between the core nucleosomes (50). Post-transcriptional modifications and gene expression through histones are often regulated by histone acetylation, methylation and phosphorylation (13). Covalent modifications, such as the acetylation of lysines, the methylation of lysines and arginines, the phosphorylation of serines and threonines, and the ubiquitination of lysines occur at the N-terminal tails of histone proteins, protruding out from the core nucleosomes (51). Histone can be mono-, di- or tri-methylated and H3 (lysines 4, 9 and 27) and H4 (lysine 20) are the most frequently methylated histones (52-53). Histone acetylation and deacetylation catalyzed by histone acetyltransferases (HATs) and histone deacetylases (HDACs) results in transcriptional activation and repression, respectively (54). However, depending on the type of amino acid and its position in the histone tail, histone methylation catalyzed by histone methyltransferases (HMTs) may result in either transcriptional activation or repression (55). Similarly, H3K9, H3K27 and H4K20 methylation results in transcriptional repression, while H3K4 methylation results in transcriptional activation (56). The overexpression of HDAC1 is associated with malignant behaviour and a poor prognosis of ICC (57). Reduced survival and cell growth arrest in the human CC cell lines with HDAC inhibitors, such as MS-275, trichostatin A, NVPLAQ824 and NVPLBH589 in a dose-dependent manner, suggests the possibility of suppressing CC with HDAC inhibitors (58-60). Furthermore, the synergistic growth inhibitory effect by the induction of apoptosis and cell cycle arrest by HDAC inhibitors with conventional cytostatic drugs, such as gemcitabine, doxorubicin, sorafenib, or bortezomib supports the therapeutic role of HDAC inhibitors in treating CC (58,60). However, the role of histone modifications in the carcinogenesis and pathogenesis of CC is not well documented; thus, further research to explore and unravel this conundrum is required in order to develop more effective diagnostic and therapeutic strategies for the treatment of CC.

miRNAs

miRNAs are small non-coding RNAs derived from polyadenylated primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs) involving RNA polymerase II and RNase III Drosha and pasha/DGCR8(61). The maturation of miRNAs is mediated by RNase III Dicer and binding with RISC (RNA-induced silencing complex). Mature miRNAs regulate gene expression at the post-transcriptional level by binding to the 3' untranslated region (3'UTR) of target mRNAs, which leads to degradation of mRNAs (62). The upregulation (overexpression) or downregulation (underexpression) of miRNAs regulates gene expression, thereby regulating tumorigenesis (Table II). The role of several miRNAs as oncogenes and tumor suppressor genes (63) and as diagnostic and prognostic markers (17) has been documented. Recently, the presence of the increased expression of oncogenic miR-24 and the decreased expression of the tumor suppression gene, multiple endocrine neoplasia type 1 (also known as menin 1 (MEN1)), and the role of miR-24 inhibition in attenuating the progression of CC has been discussed (64). Since MEN1 overexpression is associated with the decreased proliferation, angiogenesis, migration and invasion of CC, the inhibition of miR-24 resulting in an increased MEN1 protein expression may attenuate the proliferation, angiogenesis, migration and invasion of CC. Thus, targeting miR-24 may prove to be a novel therapeutic strategy.

Table II

Unique microRNAs that were identified to promote the pathogenesis of cholangiocarcinoma.

Table II

Unique microRNAs that were identified to promote the pathogenesis of cholangiocarcinoma.

Overexpressed or upregulated miRNAsTarget geneCorrelation with CC tumorigenesis(Refs.)
miR-141CLOCKTumor suppressor gene(23)
miR-200bPTPN12Tumor suppressor gene(23)
miR-21PTENTumor suppressor gene(23)
let-7aNF2Tumor suppressor gene(65)
miR-24MEN1(11q13)Tumor suppressor gene(64)
miR-26aGSK-3bTumor growth(66)
miR-429CDH-6Tumor suppressor gene(67)
miR-21, miR-31, and miR-223MultipleNo association with clinic-pathological parameters of CC(68)
Underexpressed or downregulated miRNAsTarget geneCorrelation with CC tumorigenesis(Refs.)
miR-29bMCL-1Tumor suppressor gene(65)
miR-370MAP3K8Tumor suppressor gene(65)
miR-148aDNMT-1Regulate methyltransferase(69)
miR-152DNMT-1Regulate methyltransferase(69)
miR-124SMYD3Migration and invasion of CC cells(70)
miR-214TwistOncogene(71)
miR-122, miR-145, miR-200c, miR-221, and miR-222MultipleAssociated with tumorigenesis of ICC(68)

DNA methylation, histone modification and alterations in miRNA expression are involved in the tumorigenesis of CC. Furthermore, the control of the transcription of miRNAs by DNA methylation, histone modifications and the regulation of epigenetic machinery by miRNAs suggest an association of these mechanisms in CC tumorigenesis. This suggests that the study of epigenetic alterations may provide novel and non-invasive biomarkers, strong potential screening tools, and potentially promising prognostic and diagnostic markers for CC in clinical practice (18,72-74).

lncRNAs

lncRNAs pervasively transcribed in the genome, are emerging as crucial regulators of cancer and play important roles in almost every aspect of cell biology, including tumorigenesis. lncRNAs regulate the malignant transformation of cells through their interaction with DNA, proteins and RNA. Thus, lncRNA molecular mechanisms involved in the tumorigenesis of CC may be attractive targets for therapeutic intervention in the fight against cancer (75-77). Using lncRNAs, mRNA microarrays and RT-PCR, Wang et al (78) examined the associations between the expression levels of lncRNAs and target genes, and found the upregulation of lncRNAs in ICC tissues and the downregulation of lncRNAs in non-cancerous tissues. The majority of upregulated genes are involved in carcinogenesis, hepatic system diseases and signal transductions. Furthermore, the upregulation of lncRNA CCAT1 and lncRNA AFAP1-AS1 in CC and their association with the tumor growth promotion, aggressive malignant behavior and the metastasis of CC suggest the role of lncRNAs in the pathogenesis of CC (79-80). Competing endogenous RNAs (ceRNAs) are a novel class of RNA species that can regulate miRNAs, lncRNAs, and genes that play important roles in the pathogenesis of CC (81). The interaction between lncRNA MALAT1 and miR-204 has been shown to modulate human hilar CC proliferation, migration and invasion by targeting CXCR4(82). Wang et al (76) found that cell migration and invasion in CC, by targeting IL-6 and CXCR4 via ceRNA, was regulated by lncRNA H19 and HULC, upregulated by oxidative stress. Furthermore, the co-expression of the carbamoyl-phosphate synthase 1 (CPS1) gene and its lncRNA has been shown to be associated with a poor prognosis in CC (83). Hence, the increased expression of lncRNAs in CC indicates that lncRNAs may be potential diagnostic and prognostic biomarkers for ICC; the combined assessment of lncRNA and mRNA expression levels may thus predict the survival of patients with ICC (76-80,83). Furthermore, the BRCA-1 associated protein-1 (BAP1)-dependent expression of lncRNA NEAT-1 enhancing the sensitivity to gemcitabine in CC, suggests the therapeutic role of lncRNAs (84).

Protein modifications

Post-transcriptional modifications result in the alteration of protein functions following protein expression and are associated with carcinogenesis and a number of human diseases. The wingless type (Wnt) signaling pathway plays a crucial role in the tumorigenesis of CC. Davaadorj et al (85,86) found a negative correlation between secreted frizzled-related protein-1 (SFRP1) expression and β-catenin expression in ICC and suggested that the loss of the negative regulator of the Wnt signaling pathway, SFRP1, located at chromosome 8p12e11.1, was associated with a poor prognosis of patients with ICC. Hence, the loss of SFRP1 may be a potential prognostic biomarker for ICC. These data suggest that proteomics analysis may be useful for the diagnosis and prognosis in CC. Furthermore, the differential expression of proteins during proteomics analysis may be used for the identification of the transition of the infectious liver to CC, and may thus lead to the early diagnosis and prevention of CC (87).

5. Diagnostic development

Immunohistochemistry

The immunostaining of formalin-fixed biopsied tissues with various tumor-specific markers, including CD10, CEA, CK7, CK20, CDX-2, TTF-1, ER, PR, BRST-2, ISHalbumin, Hep Par 1, Ber-Ep4, chromogranin and PSA is being used to differentiate and diagnose CC (88). However, due to the close association of the anatomic sites in the embryonic and the fetal development process of ICC from metastatic pancreatic ductal adenocarcinomas or adenocarcinoma from the upper GI tract, it has become difficult to differentiate due to the lack of tissue-specific markers (88). The inclusion of non-conventional markers (placental S100 (S100P), von Hippel-Lindau tumor suppressor (pVHL), mucin 5AC (MUC5AC) and CK17) with the existing markers may be beneficial (89). Despite the presence of various diagnostic and prognostic markers (17), early diagnosis remains a challenge and indicates thea need for more effective histological and molecular diagnostics. Nakanuma et al (49) suggested the role of S100P immunostaining in the differentiation of carcinomatous, perihilar and normal tissue. Recently, Kanzawa et al (90) discussed the role of dual immunostaining for maspin and p53 compared to S100P and p53 on cell blocks in increasing the diagnostic value of biliary brushing cytology. The role of tubulin β-III (TUBB٣) as a novel immunohistochemical marker for intrahepatic peripheral CC has also been discussed (91).

Proteomics

The serum markers, CA19-9, CA125 and CEA, have been used for the diagnosis of CC; however, their sensitivity and specificity for all histological types of CC is unclear (12). Thus, there is a need for more effective markers for early diagnosis. Patel et al (12) suggested that the addition of serum CA19-9 may aid in the differentiation of CC in patients with PSC and CC not associated with PSC. Including the proteomic-based autoantibodies analysis against heat shock protein 70, enolase 1 and ribonuclease/angiogenin Inhibitor 1 as diagnostic markers may increase the sensitivity and specificity in the early detection of CC (92,93). Similarly, using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI IMS) to reveal tissue heterogeneity in hepatic CC may aid in revealing novel relevant biomarkers for CC. Furthermore, these biomarkers may be used for diagnostic and follow-up purposes in patients who are at risk of developing CC if these are secreted and detectable in blood (94). Further, the mass spectrometry-based proteomics analysis of formalin-fixed-paraffin-embedded extrahepatic CC and the overexpression of proteins on immunohistochemical analysis with a positive rate of S100P (84%), CEAM5 (75%), MUC5A (62%), OLFM4 (60%), OAT (42%), CAD17 (41%), FABPL (38%), AOFA (30%), K1C20 (25%) and CPSM (22%) in extrahepatic CCs, but not in normal tissue, suggest the potential role of proteomics analysis in elucidating potential targets for future diagnostic biomarkers and therapy (95). Stephenson et al (96) also highlighted the role of proteomics profiling for the quantitative assessment of cell surface proteins to identify novel therapeutic targets in CC and to distinguish between distal CC and pancreatic cancers. Additionally, proteomics profiling for the identification of novel serum biomarkers may aid in differentiating between CC from benign biliary tract diseases (97). The same reports also described FAM19A5, MAGED4B, KIAA0321, RBAK and UPF3B as potential biomarkers of CC. These data suggest that proteomics profiling may be used to elucidate the potentially novel biomarker for the development of diagnosis, prognosis and therapies (98-100).

Epigenetics

Tissue heterogeneity in carcinomas confers a significant problem in early diagnosis. Although single-gene predictive assays are available, there is a need for the analysis of multiple gene loci, since the genetic, proteomic and miRNA content may vary in the biopsied sample due to tissue heterogeneity (14,49). Whole-genome sequencing and RNA sequencing may provide a comprehensive analysis of the somatic mutations and gene expression, and provide novel insights on the use of genomic data for the treatment of individual patients (14). Furthermore, genome-wide expression patterns associated with oncogenesis and the sarcomatous transdifferentiation of CC documented the up- and downregulation of tumor-related and tumor suppressor genes and proteins in human CC, including SPP1, EFNB2, E2F2, IRX3, PTTG1, PPARγ, KRT17, UCHL1, IGFBP7 and SPARC (101). This suggests that gene expression profiling for the deregulation of oncogenes, tumor suppressor genes and methylation-related genes, and their related proteins may be useful for the identification of molecular targets for the diagnosis and prognosis of CC. Furthermore, gene expression profiling may also be useful in differentiating CC from other liver masses in addition to subclassifying ICC, with better results compared to histopathological findings. Furthermore, gene profiling can also be helpful in predicting the outcome for various therapeutic modalities and patient survival (101).

6. Epigenetic therapy of cancer

Epigenetic alterations result in the inactivation and silencing of tumor suppressor genes and increased tumorigenesis. Signal transducer and activator of transcription 3 (STAT3) overexpression is associated with metastasis and poor post-surgical outcome in CC, and the inhibition of STAT3 may be a novel therapeutic target (102). Similarly, Braconi et al (103) discussed the potential of targeting the IL-6 dependent phenotype through a computational bioinformatics analysis of phenotype-based gene expression. The Wnt/catenin pathway plays a crucial role in CC tumorigenesis and the reversal of the silencing of genes involved in Wnt signaling, including SOX17, WNT3A, DKK2, SFRP1, SFRP2 and SFRP4 with DNMT inhibitor 5-aza-2'deoxycytidine in CC cells suggests the significance of targeting epigenetic mediators in CC (22). Although 5-azacitidine and 5-aza-2'deoxycytidine are US Food and Drug Administration (FDA)-approved drugs for the treatment of myelodysplastic syndrome, the in vitro and in vivo toxicity of these drugs show instability in neutral solutions (104,105). The role of the less toxic DNMT inhibitor, zebularine, a novel DNA methyltransferase inhibitor, alone or in combination with other DNMT inhibitors to enhance the re-expression of epigenetically silenced genes in cancer cells and as an inducer of cell death in CC, has been discussed (106-108). Thus, the re-activation of the silenced gene may restore gene function and its tumor-suppressing actions; thus, from this perspective, demethylating agents and HDAC inhibitors may be useful as drug candidates (22,57-60). Cell growth arrest and cell death in cancer cells can be induced by HDAC inhibitors. Another advantage with HDAC inhibitors is that normal cells are relatively resistant to them. The HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), has been approved by the FDA for T-cell cutaneous lymphoma treatment. Furthermore, the role of SAHA, valproic acid and the EZH2 inhibitor, 3-deazaneplanocin-A, as therapeutic drugs in CC, has also been discussed (109-112). Since molecular genetics, epigenetics and proteomics are evolving and promising fields in research, the study of the epigenetics of CC may enhance the effectiveness of CC therapeutics. Furthermore, targeting the specific phenotype and pathways involved in the carcinogenesis of CC, and the use of the computational bioinformatics-driven approach to discover a novel drug may prove to be beneficial (103). The key proteins regulating the epigenetic mechanisms in the pathogenesis of CC are presented in Fig. 1.

Based on the above-mentioned study results, it is imperative that despite the advancements in diagnostic tools and treatment strategies, the early diagnosis and treatment of CC remains challenging. Since the most common identified cause of CC is PSC, the diagnosis of early-stage CC requires a high index of suspicion in patients with PSC. Furthermore, due to the negative results of endoscopic brush cytology, endoscopic biopsies and imaging studies, a regular follow-up with magnetic resonance cholangopancreatography (MRCP) and advanced imaging, such as positron emission tomography (PET) scan should be carried out in all patients with PSC. Along with the PET scan, a promising imaging modality for the diagnosis of CC, namely the determination of serum levels of CA19-9, CA125 and CEA, should be carried out in an annual follow-up. Although the sensitivity and specificity of these biomarkers for all histological types of CC is unclear, CA19-9 values >100 U/ml have a 75% sensitivity and 80% specificity for CC (113). Since there are no clinical surveillance guidelines for the early detection of sporadic CC, the screening of patients with PSC with MRI, MRCP PET scan, and the determination of CA19-9 levels, is the most effective strategy for early detection. Since DNA hypermethylation is the most common aberrant epigenetic alteration in CC, the early detection of the CC can also be facilitated by DNA methylation assay of the bile fluids, including a panel of CCND2, CDH13, GRIN2B, Runt-related transcription factor 3 (RUNX3) and Twist-related protein 1 (TWIST1). This method for the detection of CC has a sensitivity of 83% and a specificity of 100% (13,114,115). Screening for the genetic and epigenetic alterations in the precursor lesions, including intraductal papillary neoplasm of the bile duct (IPNB) and biliary intraepithelial neoplasia (BilIN), as discussed by Ettel et al, may also be beneficial (116). The diagnostic and treatment challenges are also due to the heterogeneity of clinical presentations, which may be due to the origin of CC from topographically heterogeneous cholangiocytes. In cases of ICC, clinical presentation is highly heterogenous (mass-forming type, infiltrative type, etc.) and genetic alterations in cases with mass-forming type (ICC) have been shown to be similar to those in cases with hepatocellular carcinoma, and in cases with infiltrative type, genetic alterations have been shown to be similar with those in cases with perihilar CC. The clinicopathological, immunohistochemical and molecular profile similarity of Muc-ICCs with hilar CCs (from mucin-producing cholangiocytes) and of mixed-ICCs with CLCs (thought to be of HPC origin) and varying degrees of biliary epithelial differentiation has been reported (117,118). Due to the complexity of origin and clinical presentation, the treatment of CC as a whole is difficult, and there is a need to focus on the site of origin for treatment. In other words, individualized treatment should be preferred for the treatment of CC. In early-stage CC (perihilar CCA), liver transplantation with pre-operative radiation and chemotherapy and exploratory laparotomy needs to be performed to ensure the absence of metastases as a viable therapeutic option, whereas patients with ICC can be treated surgically (113,119).

7. Conclusions

CCs are rare notoriously malignant tumors with a very poor survival rates even following surgical resection. The delayed presentation of the tumor is the main reason for the delayed diagnosis and poor survival. Currently, the available panel of tissue and serum biomarkers can diagnose the tumor at a late stage only, and is lacking any modalities for diagnosis at an early stage. Thus, there is a need for the identification of more effective diagnostic biomarkers for the early diagnosis of CC. Recent advances in immunohistochemistry and molecular genetics paved the way for improved diagnostics. The role of epigenetic and proteomic alterations in the pathogenesis of CC has been documented, and these alterations may serve as the early diagnostic and prognostic markers for CC. Furthermore, the role of epigenetic therapy with DNMT and HDAC inhibitors discussed in the literature are in the early stages of clinical trials. The findings of various studies discussed in this review suggest that epigenetic and proteomic alterations may serve as more effective diagnostic markers for CC in the early stages, and epigenetic therapy may be beneficial for the treatmetn of CC. However, further research is required to investigate the initial events occurring in CC.

Acknowledgements

Not applicable.

Funding

This study was supported by Creighton University LB692 Clinical and Translational Research Grant to CSB.

Availability of data and materials

Not applicable.

Authors' contributions

VR, CSB and DKA were involved in the conception and design of the study. Drafting of the article was performed by VR. Critical revisions in the article were carried out by VR and CSB. All authors have read complete manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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July 2019
Volume 1 Issue 4

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APA
Rai, V., Boosani, C.S., & Agrawal, D.K. (2019). The promising role of epigenetic mediators and microRNAs in the early diagnosis of cholangiocarcinoma (Review). World Academy of Sciences Journal, 1, 165-176. https://doi.org/10.3892/wasj.2019.18
MLA
Rai, V., Boosani, C. S., Agrawal, D. K."The promising role of epigenetic mediators and microRNAs in the early diagnosis of cholangiocarcinoma (Review)". World Academy of Sciences Journal 1.4 (2019): 165-176.
Chicago
Rai, V., Boosani, C. S., Agrawal, D. K."The promising role of epigenetic mediators and microRNAs in the early diagnosis of cholangiocarcinoma (Review)". World Academy of Sciences Journal 1, no. 4 (2019): 165-176. https://doi.org/10.3892/wasj.2019.18