Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma

  • Authors:
    • Costansia Bureta
    • Yoshinobu Saitoh
    • Hiroto Tokumoto
    • Hiromi Sasaki
    • Shingo Maeda
    • Satoshi Nagano
    • Setsuro Komiya
    • Noboru Taniguchi
    • Takao Setoguchi
  • View Affiliations

  • Published online on: April 4, 2019     https://doi.org/10.3892/or.2019.7100
  • Pages: 3404-3412
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Abstract

The treatment of glioblastoma is a critical health issue, owing to its resistance to chemotherapy. The current standard of treatment is surgical resection, followed by adjuvant radiotherapy and temozolomide treatment. Long‑term local treatment of glioblastoma is rarely achieved and the majority of the patients undergo relapse. Resistance to temozolomide emerges from numerous signalling pathways that are altered in glioblastoma, including the Hedgehog signalling pathway. Hence, further research is required to identify effective treatment modalities. We investigated the effect of vismodegib, arsenic trioxide and temozolomide on glioblastoma in vitro and in vivo to apply our findings to the clinical setting. WST‑1 assay revealed that glioblastoma proliferation was inhibited following treatment with these drugs either in single or in combination; this synergistic effect was confirmed by CalcuSyn software. Western blot analysis revealed an increase in the expression of cleaved caspase‑3 and γH2AX. Furthermore, there was marked inhibition and decreased tumour growth in mice that received combination therapy, unlike those that received single agent or vehicle treatment. Our results revealed that the combination of arsenic trioxide/vismodegib and temozolomide may be an attractive therapeutic method for the treatment of glioblastoma.
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June 2019
Volume 41 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Bureta, C., Saitoh, Y., Tokumoto, H., Sasaki, H., Maeda, S., Nagano, S. ... Setoguchi, T. (2019). Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma. Oncology Reports, 41, 3404-3412. https://doi.org/10.3892/or.2019.7100
MLA
Bureta, C., Saitoh, Y., Tokumoto, H., Sasaki, H., Maeda, S., Nagano, S., Komiya, S., Taniguchi, N., Setoguchi, T."Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma". Oncology Reports 41.6 (2019): 3404-3412.
Chicago
Bureta, C., Saitoh, Y., Tokumoto, H., Sasaki, H., Maeda, S., Nagano, S., Komiya, S., Taniguchi, N., Setoguchi, T."Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma". Oncology Reports 41, no. 6 (2019): 3404-3412. https://doi.org/10.3892/or.2019.7100