microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

  • Authors:
    • Jian Hua Mao
    • Rong Ping Zhou
    • Ai Fen Peng
    • Zhi Li Liu
    • Shan Hu Huang
    • Xin Hua Long
    • Yong Shu
  • View Affiliations

  • Published online on: August 16, 2012     https://doi.org/10.3892/ol.2012.863
  • Pages: 1125-1129
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Abstract

microRNAs are involved in different cancer-related processes. miR-195, one of the miR-16/15/195/424/497 family members, has been shown to act as a tumor suppressor during tumorigenesis. However, the function of miR-195 in osteosarcoma is still unclear. In our study, the miR-195 expression level was upregulated in osteosarcoma cells, by transfection with miR-195, and the fatty acid synthase (FASN) mRNA and protein expression levels were measured by RT-PCR and western blotting. Cell migration and invasion was measured using wound healing migration and Transwell invasion assays. We found that the upregulation of miR-195 greatly decreased cell invasion and the migration of U2OS. We also identified that FASN may be a direct target of miR-195 by the luciferase activity assay. These findings provide evidence that miR-195 plays a key role in inhibiting osteosarcoma cell migration and invasion through targeting FASN, and strongly suggest that exogenous miR-195 may have therapeutic value in treating osteosarcoma.

References

1. 

L MirabelloRJ TroisiSA SavageOsteosarcoma incidence and survival rates from 1973 to 2004: data from the Surveillance, Epidemiology, and End Results ProgramCancer11515311543200910.1002/cncr.2412119197972

2. 

VO LewisWhat’s new in musculoskeletal oncologyJ Bone Joint Surg Am91154615562009

3. 

PA MeyersCL SchwartzM KrailoOsteosarcoma: a randomized, prospective trial of the addition of ifosfamide and/or muramyl tripeptide to cisplatin, doxorubicin, and high-dose methotrexateJ Clin Oncol2320042011200510.1200/JCO.2005.06.03115774791

4. 

Y ChoGH JungSH ChungJY KimY ChoiJD KimLong-term survivals of stage IIb osteosarcoma: a 20-year experience in a single institutionClin Orthop Surg34854201121369478

5. 

H TsuchiyaK TomitaY MoriCaffeine-assisted chemotherapy and minimized tumor excision for nonmetastatic osteosarcomaAnticancer Res1865766619989584049

6. 

T BollingP SchullerB DistelmaierPerioperative high-dose rate brachytherapy using a bendy applicator (flab): treatment results of 74 patientsAnticancer Res2838853890200819192645

7. 

N MarinaM GebhardtL TeotR GorlickBiology and therapeutic advances for pediatric osteosarcomaOncologist9422441200410.1634/theoncologist.9-4-42215266096

8. 

T WadaK IsuN TakedaM UsuiS IshiiS YamawakiA preliminary report of neoadjuvant chemotherapy NSH-7 study in osteosarcoma: preoperative salvage chemotherapy based on clinical tumor response and the use of granulocyte colony-stimulating factorOncology53221227199610.1159/000227564

9. 

CS YehJY WangTL ChengCH JuanCH WuSR LinFatty acid metabolism pathway play an important role in carcinogenesis of human colorectal cancers by Microarray-Bioinformatics analysisCancer Lett233297308200610.1016/j.canlet.2005.03.05015885896

10. 

D HessRA IgalGenistein downregulates de novo lipid synthesis and impairs cell proliferation in human lung cancer cellsExp Biol Med (Maywood)236707713201110.1258/ebm.2011.01026521565896

11. 

PL AloM AminiF PiroImmunohistochemical expression and prognostic significance of fatty acid synthase in pancreatic carcinomaAnticancer Res2725232527200717695548

12. 

K WalterSM HongS NyhanSerum fatty acid synthase as a marker of pancreatic neoplasiaCancer Epidemiol Biomarkers Prev1823802385200910.1158/1055-9965.EPI-09-014419723916

13. 

Y OkawaT HideshimaH IkedaFatty acid synthase is a novel therapeutic target in multiple myelomaBr J Haematol141659671200810.1111/j.1365-2141.2008.07114.x

14. 

T MigitaS RuizA FornariFatty acid synthase: a metabolic enzyme and candidate oncogene in prostate cancerJ Natl Cancer Inst101519532200910.1093/jnci/djp03019318631

15. 

H OritaJ CoulterE TullyFP KuhajdaE GabrielsonInhibiting fatty acid synthase for chemoprevention of chemically induced lung tumorsClin Cancer Res1424582464200810.1158/1078-0432.CCR-07-417718413838

16. 

DT ColemanR BigelowJA CardelliInhibition of fatty acid synthase by luteolin post-transcriptionally down-regulates c-Met expression independent of proteosomal/lysosomal degradationMol Cancer Ther8214224200910.1158/1535-7163.MCT-08-072219139131

17. 

GE SaatiMC ArcherInhibition of fatty acid synthase and Sp1 expression by 3,3′-diindolylmethane in human breast cancer cellsNutr Cancer637907942011

18. 

M NotarnicolaS PisantiV TutinoEffects of olive oil polyphenols on fatty acid synthase gene expression and activity in human colorectal cancer cellsGenes Nutr66369201110.1007/s12263-010-0177-721437031

19. 

M NotarnicolaC MessaMG RefoloV TutinoA MiccolisMG CarusoPolyunsaturated fatty acids reduce fatty acid synthase and hydroxy-methyl-glutaryl CoA-reductase gene expression and promote apoptosis in HepG2 cell lineLipids Health Dis1010201110.1186/1476-511X-10-1021244676

20. 

KG ZecchinFA RossatoHF RaposoInhibition of fatty acid synthase in melanoma cells activates the intrinsic pathway of apoptosisLab Invest91232240201110.1038/labinvest.2010.15720805790

21. 

MA CarvalhoKG ZecchinF SeguinFatty acid synthase inhibition with Orlistat promotes apoptosis and reduces cell growth and lymph node metastasis in a mouse melanoma modelInt J Cancer12325572565200810.1002/ijc.2383518770866

22. 

S MurataK YanagisawaK FukunagaFatty acid synthase inhibitor cerulenin suppresses liver metastasis of colon cancer in miceCancer Sci10118611865201010.1111/j.1349-7006.2010.01596.x20491775

23. 

VN KimJ HanMC SiomiBiogenesis of small RNAs in animalsNat Rev Mol Cell Biol10126139200910.1038/nrm263219165215

24. 

DP BartelMicroRNAs: target recognition and regulatory functionsCell136215233200910.1016/j.cell.2009.01.00219167326

25. 

MA Valencia-SanchezJ LiuGJ HannonR ParkerControl of translation and mRNA degradation by miRNAs and siRNAsGenes Dev20515524200610.1101/gad.139980616510870

26. 

GA CalinC SevignaniCD DumitruHuman microRNA genes are frequently located at fragile sites and genomic regions involved in cancersProc Natl Acad Sci USA10129993004200410.1073/pnas.030732310114973191

27. 

X WangJ WangH MaJ ZhangX ZhouDownregulation of miR-195 correlates with lymph node metastasis and poor prognosis in colorectal cancerMed Oncol201121390519

28. 

X BaiD MaA LiuRheb activates mTOR by antagonizing its endogenous inhibitor, FKBP38Science318977980200710.1126/science.114737917991864

29. 

T XuY ZhuY XiongYY GeJP YunSM ZhuangMicroRNA-195 suppresses tumorigenicity and regulates G1/S transition of human hepatocellular carcinoma cellsHepatology50113121200910.1002/hep.2291919441017

30. 

PS SoonLJ TaconAJ GillmiR-195 and miR-483-5p identified as predictors of poor prognosis in adrenocortical cancerClin Cancer Res1576847692200910.1158/1078-0432.CCR-09-158719996210

31. 

TS WongXB LiuBY WongRW NgAP YuenWI WeiMature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongueClin Cancer Res1425882592200810.1158/1078-0432.CCR-07-066618451220

32. 

L LiuL ChenY XuR LiX DumicroRNA-195 promotes apoptosis and suppresses tumorigenicity of human colorectal cancer cellsBiochem Biophys Res Commun400236240201010.1016/j.bbrc.2010.08.04620727858

33. 

D LiY ZhaoC LiuAnalysis of MiR-195 and MiR-497 expression, regulation and role in breast cancerClin Cancer Res1717221730201110.1158/1078-0432.CCR-10-180021350001

34. 

B BrennerMB HoshenO PurimMicroRNAs as a potential prognostic factor in gastric cancerWorld J Gastroenterol1739763985201110.3748/wjg.v17.i35.397622046085

35. 

HM HeneghanN MillerR KellyJ NewellMJ KerinSystemic miR-195 differentiates breast cancer from other malignancies and is a potential biomarker for detecting noninvasive and early stage diseaseOncologist15673682201010.1634/theoncologist.2010-010320576643

36. 

HM HeneghanN MillerAJ LoweryKJ SweeneyJ NewellMJ KerinCirculating microRNAs as novel minimally invasive biomarkers for breast cancerAnn Surg251499505201010.1097/SLA.0b013e3181cc939f20134314

37. 

BN HannafonP SebastianiA de las MorenasJ LuCL RosenbergExpression of microRNA and their gene targets are dysregulated in preinvasive breast cancerBreast Cancer Res13R24201110.1186/bcr283921375733

38. 

H ZhangSB SuQM ZhouYY LuDifferential expression profiles of microRNAs between breast cancer cells and mammary epithelial cellsAi Zheng28493499200919624877

39. 

DL ZanetteF RivadaviaGA MolfettamiRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemiaBraz J Med Biol Res4014351440200710.1590/S0100-879X200700110000317934639

40. 

WY WuXY XueZJ ChenPotentially predictive microRNAs of gastric cancer with metastasis to lymph nodeWorld J Gastroenterol1736453651201110.3748/wjg.v17.i31.364521987613

41. 

M SelbachB SchwanhausserN ThierfelderZ FangR KhaninN RajewskyWidespread changes in protein synthesis induced by microRNAsNature4555863200810.1038/nature0722818668040

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November 2012
Volume 4 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Mao, J.H., Zhou, R.P., Peng, A.F., Liu, Z.L., Huang, S.H., Long, X.H., & Shu, Y. (2012). microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN. Oncology Letters, 4, 1125-1129. https://doi.org/10.3892/ol.2012.863
MLA
Mao, J. H., Zhou, R. P., Peng, A. F., Liu, Z. L., Huang, S. H., Long, X. H., Shu, Y."microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN". Oncology Letters 4.5 (2012): 1125-1129.
Chicago
Mao, J. H., Zhou, R. P., Peng, A. F., Liu, Z. L., Huang, S. H., Long, X. H., Shu, Y."microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN". Oncology Letters 4, no. 5 (2012): 1125-1129. https://doi.org/10.3892/ol.2012.863