microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

  • Authors:
    • Jian Hua Mao
    • Rong Ping Zhou
    • Ai Fen Peng
    • Zhi Li Liu
    • Shan Hu Huang
    • Xin Hua Long
    • Yong Shu
  • View Affiliations

  • Published online on: August 16, 2012     https://doi.org/10.3892/ol.2012.863
  • Pages: 1125-1129
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microRNAs are involved in different cancer-related processes. miR-195, one of the miR-16/15/195/424/497 family members, has been shown to act as a tumor suppressor during tumorigenesis. However, the function of miR-195 in osteosarcoma is still unclear. In our study, the miR-195 expression level was upregulated in osteosarcoma cells, by transfection with miR-195, and the fatty acid synthase (FASN) mRNA and protein expression levels were measured by RT-PCR and western blotting. Cell migration and invasion was measured using wound healing migration and Transwell invasion assays. We found that the upregulation of miR-195 greatly decreased cell invasion and the migration of U2OS. We also identified that FASN may be a direct target of miR-195 by the luciferase activity assay. These findings provide evidence that miR-195 plays a key role in inhibiting osteosarcoma cell migration and invasion through targeting FASN, and strongly suggest that exogenous miR-195 may have therapeutic value in treating osteosarcoma.



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November 2012
Volume 4 Issue 5

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Online ISSN:1792-1082

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Copy and paste a formatted citation
Mao, J.H., Zhou, R.P., Peng, A.F., Liu, Z.L., Huang, S.H., Long, X.H., & Shu, Y. (2012). microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN. Oncology Letters, 4, 1125-1129. https://doi.org/10.3892/ol.2012.863
Mao, J. H., Zhou, R. P., Peng, A. F., Liu, Z. L., Huang, S. H., Long, X. H., Shu, Y."microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN". Oncology Letters 4.5 (2012): 1125-1129.
Mao, J. H., Zhou, R. P., Peng, A. F., Liu, Z. L., Huang, S. H., Long, X. H., Shu, Y."microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN". Oncology Letters 4, no. 5 (2012): 1125-1129. https://doi.org/10.3892/ol.2012.863