Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells

  • Authors:
    • Shusuke Yasuda
    • Mano Horinaka
    • Toshiyuki Sakai
  • View Affiliations

  • Published online on: August 14, 2019     https://doi.org/10.3892/ol.2019.10739
  • Pages: 4253-4261
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Abstract

Sulforaphane and Lactobacilli induce apoptosis in several cancer cells. Sulforaphane, a dietary isothiocyanate, is an attractive agent due to its potent anticancer effects. Sulforaphane suppresses the proliferation of various cancer cells in vitro and in vivo. The present study investigated the effect of sulforaphane and a co‑culture with Lactobacillus‑treated peripheral blood mononuclear cells (PBMCs) in human colon cancer cells. The combination markedly induced apoptosis in human colon cancer HCT116 and SW480 cells. A pan‑caspase inhibitor markedly inhibited apoptosis, and a tumor necrosis factor (TNF) receptor/Fc chimera partially inhibited apoptosis in both cells. The amount of TNFα secretion in the culture supernatant was significantly increased by co‑culture with Lactobacillus‑treated normal human PBMCs. On the other hand, the expression of cellular inhibitor of apoptosis‑2 (cIAP‑2), an anti‑apoptotic protein, was increased by co‑culture with Lactobacillus‑treated PBMCs in colon cancer cells, but sulforaphane treatment significantly suppressed the induction of cIAP‑2. The present results revealed that sulforaphane enhances apoptosis in human colon cancer cells under co‑culture with Lactobacillus‑treated PBMCs via the TNFα signaling pathway.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Yasuda, S., Horinaka, M., & Sakai, T. (2019). Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells. Oncology Letters, 18, 4253-4261. https://doi.org/10.3892/ol.2019.10739
MLA
Yasuda, S., Horinaka, M., Sakai, T."Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells". Oncology Letters 18.4 (2019): 4253-4261.
Chicago
Yasuda, S., Horinaka, M., Sakai, T."Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells". Oncology Letters 18, no. 4 (2019): 4253-4261. https://doi.org/10.3892/ol.2019.10739