Open Access

ILK predicts the efficacy of chemoradiotherapy and the prognosis of patients with esophageal squamous cell carcinoma

  • Authors:
    • Xiao‑Li Ma
    • Hua Yao
    • Xiao Wang
    • Yu Wei
    • Lei‑Yu Cao
    • Qian Zhang
    • Li Zhang
  • View Affiliations

  • Published online on: August 22, 2019     https://doi.org/10.3892/ol.2019.10768
  • Pages: 4114-4125
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Isobaric tags for relative and absolute quantitation (iTRAQ) technology was adopted to screen differentially‑expressed proteins in the serum that predict the effects of chemoradiotherapy on esophageal squamous cell carcinoma (ESCC). Thus, significantly related proteins can be functionally identified at the cellular level. A total of 60 patients diagnosed with locally advanced and advanced ESCC were recruited and treated with chemoradiotherapy. The iTRAQ technique was used to screen serum differentially expressed proteins associated with chemoradiotherapeutic efficacy. Functional identification of significantly related proteins was performed at the cellular level. Cell proliferation was detected using MTT, clonogenic and fluorescence assays, and apoptosis was assessed using flow cytometry. Transwell and wound‑healing assays were used to detect the invasion and migration properties of cancer cells. Proteomics results revealed that prior to chemoradiotherapy, the expression level of integrin‑linked kinase (ILK) was significantly upregulated in patients with ESCC, compared with that of the control group [ratio (r)=4.386; P<0.05], and significantly downregulated in the chemoradiotherapy‑sensitive group, compared with the chemoradiotherapy‑resistant group (r=0.587; P<0.05). At the cellular level, the proliferation rate of cells in the experimental group was significantly inhibited (P<0.05), and the number of cell colonies was decreased (P<0.01), while the number of apoptotic cells was significantly increased (P<0.01). The invasive ability of TE‑1 cells in the shILK group was significantly inhibited (P<0.01), and the migration rate was significantly retarded at 8 and 24 h (P<0.01). The present study highlighted the potential value of ILK in predicting the efficacy of chemoradiotherapeutic treatment in patients with ESCC, and that ILK gene‑silencing inhibits the progression of ESCC.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Ma, X., Yao, H., Wang, X., Wei, Y., Cao, L., Zhang, Q., & Zhang, L. (2019). ILK predicts the efficacy of chemoradiotherapy and the prognosis of patients with esophageal squamous cell carcinoma. Oncology Letters, 18, 4114-4125. https://doi.org/10.3892/ol.2019.10768
MLA
Ma, X., Yao, H., Wang, X., Wei, Y., Cao, L., Zhang, Q., Zhang, L."ILK predicts the efficacy of chemoradiotherapy and the prognosis of patients with esophageal squamous cell carcinoma". Oncology Letters 18.4 (2019): 4114-4125.
Chicago
Ma, X., Yao, H., Wang, X., Wei, Y., Cao, L., Zhang, Q., Zhang, L."ILK predicts the efficacy of chemoradiotherapy and the prognosis of patients with esophageal squamous cell carcinoma". Oncology Letters 18, no. 4 (2019): 4114-4125. https://doi.org/10.3892/ol.2019.10768