Open Access

All‑trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line

  • Authors:
    • Ruizhi Wang
    • Chongxiao Liu
  • View Affiliations

  • Published online on: July 31, 2019     https://doi.org/10.3892/ol.2019.10691
  • Pages: 3646-3654
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The poor therapeutic effect of the current treatments for malignant glioma may be attributed to glioma stem cells (GSCs), which have been demonstrated to divide symmetrically. All‑trans retinoic acid (ATRA)‑induced differentiation is considered to target GSCs and has been reported to have the capability of eradicating cancer stem cells in specific malignancies. The aim of the present study was to investigate the effects of ATRA on the division mode of GSCs isolated from the U87MG glioblastoma cell line of unknown origin. The expressions of the GSC markers CD133 and nestin were detected using immunocytochemistry to identify GSCs. In addition, the differentiation potency of these GSCs was observed by detecting the expression of glial fibrillary acidic protein, β‑tubulin III and galactosylceramidase using immunofluorescent staining. The Numb protein distribution was analyzed in two daughter cells following a GSC division. The results of the present study demonstrated that Numb protein is symmetrically segregated into two daughter cells during GSC division. Furthermore, the present study demonstrated that treatment with ATRA increased the asymmetric cell division of GSCs. In conclusion, these results suggest a therapeutic effect from ATRA‑induced asymmetric division of GSCs from the U87MG cell line.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Wang, R., & Wang, R. (2019). All‑trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line. Oncology Letters, 18, 3646-3654. https://doi.org/10.3892/ol.2019.10691
MLA
Wang, R., Liu, C."All‑trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line". Oncology Letters 18.4 (2019): 3646-3654.
Chicago
Wang, R., Liu, C."All‑trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line". Oncology Letters 18, no. 4 (2019): 3646-3654. https://doi.org/10.3892/ol.2019.10691