Downregulation of thymopoietin by miR‑139‑5p suppresses cell proliferation and induces cell cycle arrest/apoptosis in pancreatic ductal adenocarcinoma
- Huadong Zhou
- Linfei Zhang
- Huahua Tu
Affiliations: Department of Hepatobiliary Pancreatic Surgery, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
- Published online on: July 29, 2019 https://doi.org/10.3892/ol.2019.10679
Copyright: © Zhou
et al. This is an open access article distributed under the
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MicroRNAs (miRNAs) serve a pivotal role in tumor development and progression, in which miRNA (miR)‑139‑5p functions as a tumor suppressor. However, the functions and mechanisms of miR‑139‑5p in pancreatic ductal adenocarcinoma (PDAC) remain unclear. In the present study, it was found that miR‑139‑5p was markedly decreased in PDAC tissues and cell lines. Noticeably, thymopoietin (TMPO) was predicted and confirmed as a direct target of miR‑139‑5p using a luciferase reporter system. The expression level of miR‑139‑5p was inversely associated with the expression of TMPO in PDAC specimens. A series of gain‑of‑function assays elucidated that the overexpression of miR‑139‑5p suppressed cell proliferation, and induced cell cycle arrest and cell apoptosis, determined with a Cell Counting Kit‑8, colony formation assays and flow cytometry, respectively. Furthermore, the re‑expression of TMPO eliminated the effects of miR‑139‑5p on cell proliferation, cell cycle progression and apoptosis. In summary, these findings demonstrated that miR‑139‑5p may be a tumor suppressor in PDAC, which may be useful in developing promising therapies for PDAC.