Open Access

Artemisinin induced reversal of EMT affects the molecular biological activity of ovarian cancer SKOV3 cell lines

  • Authors:
    • Weichen Liang
    • Jian Liu
    • Huazhang Wu
    • Xuxu Qiao
    • Xiang Lu
    • Yonghong Liu
    • Hong Zhu
    • Ling Ma
  • View Affiliations

  • Published online on: July 11, 2019     https://doi.org/10.3892/ol.2019.10608
  • Pages: 3407-3414
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Accumulating evidence suggests that celecoxib and artemisinin could mediate ovarian cancer development and metastasis. The present study investigated the effects of celecoxib and artemisinin on the epithelial‑mesenchymal transition (EMT) characteristics of the human ovarian epithelial adenocarcinoma cell line, SKOV3. SKOV3 cells were incubated with celecoxib (10 µM) for different periods of time to establish an EMT cell model. Subsequently, artemisinin (20, 40 and 80 µM) was used to establish a cell model of the reverse process, mesenchymal‑epithelial transition (MET). Cell proliferation, metastasis, invasiveness and the expression of vimentin and E‑cadherin were measured using Cell Counting Kit‑8, wound healing assay, western blotting, flow cytometry and immunofluorescence. The EMT cell model exhibited enhanced proliferative capacity, increased migration, increased vimentin expression and decreased E‑cadherin expression. By contrast, artemisinin decreased proliferative capacity, decreased migration, decreased vimentin expression and increased E‑cadherin expression of EMT model cells, indicating that MET was induced. These results demonstrated that artemisinin may reverse celecoxib‑induced epithelial‑mesenchymal transition in SKOV3 cells.
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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Liang, W., Liu, J., Wu, H., Qiao, X., Lu, X., Liu, Y. ... Ma, L. (2019). Artemisinin induced reversal of EMT affects the molecular biological activity of ovarian cancer SKOV3 cell lines. Oncology Letters, 18, 3407-3414. https://doi.org/10.3892/ol.2019.10608
MLA
Liang, W., Liu, J., Wu, H., Qiao, X., Lu, X., Liu, Y., Zhu, H., Ma, L."Artemisinin induced reversal of EMT affects the molecular biological activity of ovarian cancer SKOV3 cell lines". Oncology Letters 18.3 (2019): 3407-3414.
Chicago
Liang, W., Liu, J., Wu, H., Qiao, X., Lu, X., Liu, Y., Zhu, H., Ma, L."Artemisinin induced reversal of EMT affects the molecular biological activity of ovarian cancer SKOV3 cell lines". Oncology Letters 18, no. 3 (2019): 3407-3414. https://doi.org/10.3892/ol.2019.10608