Open Access

Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non‑small cell lung cancer

  • Authors:
    • Dong‑Seok Kim
    • Nyoun Soo Kwon
    • Hye‑Young Yun
  • View Affiliations

  • Published online on: July 22, 2019     https://doi.org/10.3892/ol.2019.10648
  • Pages: 3388-3398
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Leucine rich repeat LGI family member 3 (LGI3) is a member of the LGI protein family. Our previous studies reported that LGI3 was expressed in adipose tissues, brain and skin, where it served roles as a multifunctional cytokine and pro‑inflammatory adipokine. It was hypothesized that LGI3 may be involved in cytokine networks in cancer. The present study aimed to analyze differentially expressed genes in non‑small cell lung cancer (NSCLC) tissues and NSCLC cohort data, to evaluate the prognostic role of LGI3. Expression microarray and NSCLC cohort data were statistically analyzed by bioinformatic methods, and protein‑protein interactions, functional enrichment and pathway, gene coexpression network (GCN) and prognostic association analyses were performed. The results demonstrated that the expression levels of LGI3 and its receptor a disintegrin and metalloproteinase domain‑containing protein 22 were significantly decreased in NSCLC tissues. A total of two upregulated genes and 11 downregulated genes in NSCLC tissues were identified as LGI3‑regulated genes. Protein‑protein interaction network analysis demonstrated that all LGI3‑regulated genes that were altered in NSCLC were involved in a protein‑protein interaction network cluster. Functional enrichment, Kyoto Encyclopedia of Genes and Genomes pathway and GCN analyses demonstrated the association of these genes with the immune and inflammatory responses, angiogenesis, the tumor necrosis factor pathway, and chemokine and peroxisome proliferator‑activated receptor signaling pathways. Analysis of NSCLC cohorts revealed that low expression levels of LGI3 was significantly associated with poor prognosis of NSCLC. Analysis of the somatic mutations of the LGI3 gene in NSCLC revealed that the amino acid residues altered in NSCLC included two single nucleotide polymorphism sites and three phylogenetically coevolved amino acid residues. Taken together, these results suggest that LGI3 may be a potential prognostic marker of NSCLC.
View Figures
View References

Related Articles

Journal Cover

September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kim, D., Kwon, N.S., & Yun, H. (2019). Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non‑small cell lung cancer. Oncology Letters, 18, 3388-3398. https://doi.org/10.3892/ol.2019.10648
MLA
Kim, D., Kwon, N. S., Yun, H."Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non‑small cell lung cancer". Oncology Letters 18.3 (2019): 3388-3398.
Chicago
Kim, D., Kwon, N. S., Yun, H."Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non‑small cell lung cancer". Oncology Letters 18, no. 3 (2019): 3388-3398. https://doi.org/10.3892/ol.2019.10648