MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5

  • Authors:
    • Chan Li
    • Hua Yan
    • Jie Yin
    • Jian Ma
    • Ang Liao
    • Shengyou Yang
    • Lijuan Wang
    • Yongxiang Huang
    • Chon Lin
    • Zhiqiang Dong
    • Bo Yang
    • Ting Cao
    • Guo Liu
    • Lv Wang
  • View Affiliations

  • Published online on: July 25, 2019     https://doi.org/10.3892/ol.2019.10667
  • Pages: 3367-3372
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Abstract

Abnormal expression of microRNA (miR)‑21 has been reported in various types of cancers. However, the role and mechanism of miR‑21 remain to be elucidated in acute myeloid leukemia (AML). In the present study, it was observed that miR‑21 was upregulated and Krüppel‑like factor 5 (KLF5) was downregulated in AML cells compared with normal bone marrow cells. Dual luciferase reporter assays revealed that KLF5 was a direct target of miR‑21. Indeed, miR‑21 overexpression resulted in a downregulation of KLF5 expression, while miR‑21 inhibition had the opposite effect in AML cells. In addition, miR‑21 overexpression promoted the proliferation of AML cells in vitro. Notably, using a mouse xenograft model, miR‑21 overexpression was demonstrated to result in enhanced tumor growth and suppressed KLF5 expression in the xenograft tumors in vivo. In conclusion, the present results indicated that miR‑21 promoted proliferation through directly regulating KLF5 expression in AML cells. miR‑21 may thus serve as an oncogene in AML, providing a potential target for AML therapy.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Li, C., Yan, H., Yin, J., Ma, J., Liao, A., Yang, S. ... Wang, L. (2019). MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5. Oncology Letters, 18, 3367-3372. https://doi.org/10.3892/ol.2019.10667
MLA
Li, C., Yan, H., Yin, J., Ma, J., Liao, A., Yang, S., Wang, L., Huang, Y., Lin, C., Dong, Z., Yang, B., Cao, T., Liu, G., Wang, L."MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5". Oncology Letters 18.3 (2019): 3367-3372.
Chicago
Li, C., Yan, H., Yin, J., Ma, J., Liao, A., Yang, S., Wang, L., Huang, Y., Lin, C., Dong, Z., Yang, B., Cao, T., Liu, G., Wang, L."MicroRNA‑21 promotes proliferation in acute myeloid leukemia by targeting Krüppel‑like factor 5". Oncology Letters 18, no. 3 (2019): 3367-3372. https://doi.org/10.3892/ol.2019.10667