Open Access

MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway

  • Authors:
    • Jianying Cui
    • Guodong Pan
    • Qingjuan He
    • Lizhi Yin
    • Rui Guo
    • Hongmei Bi
  • View Affiliations

  • Published online on: July 15, 2019     https://doi.org/10.3892/ol.2019.10619
  • Pages: 2931-2938
  • Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The specific function of microRNA-545 (miR-545) has been reported to regulate the development of human cancers. However, the effect of miR-545 is still unclear in non‑small cell lung cancer (NSCLC). Hence, this study explored the molecular mechanism of miR-545 in NSCLC. The expression levels of miR-545 and ZEB2 were measured through reverse transcription‑quantitative polymerase chain reaction (RT-qPCR) assay. The protein expression was detected by western blotting. Dual luciferase assay was applied to evaluate the relationship between miR-545 and zinc finger E-box‑binding homeobox 2 (ZEB2). MTT and Transwell assays were used to investigate the function of miR-545 in NSCLC. The expression of miR-545 was decreased in NSCLC tissues. The overexpression of miR-545 suppressed the migration, invasion and proliferation of NSCLC cells. Furthermore, ZEB2 was a direct target gene of miR-545. The knockout of ZEB2 suppressed the development of NSCLC. miR-545 inhibited the progression of NSCLC through targeting ZEB2. Moreover, miR-545 repressed the development of NSCLC via blocking EMT and Wnt/β-catenin pathway. In conclusion, miR-545 inhibited the progression of NSCLC through targeting ZEB2 and blocking EMT and Wnt/β-catenin pathway.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., & Bi, H. (2019). MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway. Oncology Letters, 18, 2931-2938. https://doi.org/10.3892/ol.2019.10619
MLA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18.3 (2019): 2931-2938.
Chicago
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18, no. 3 (2019): 2931-2938. https://doi.org/10.3892/ol.2019.10619