Open Access

Fibulin-2 inhibits development of gastric cancer by downregulating β-catenin

  • Authors:
    • Hongping Ma
    • Changhong Lian
    • Yingming Song
  • View Affiliations

  • Published online on: July 10, 2019     https://doi.org/10.3892/ol.2019.10599
  • Pages: 2799-2804
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the expression of fibulin-2 and β-catenin in gastric cancer tissues and the association to mutual regulation. Forty-nine cases of gastric cancer specimens obtained via surgical resection in the Pathology Department of Heping Hospital affiliated to Changzhi Medical College from March 2013 to August 2017 were collected. The expression levels of fibulin-2 and β-catenin in 49 cases of gastric cancer and para-carcinoma tissues were detected via quantitative polymerase chain reaction and immunohistochemistry. The correlation of expression of fibulin-2 and β-catenin proteins in gastric cancer was detected via Spearman's analysis. The correlation of fibulin-2 and β-catenin protein expression with clinicopathological indexes of patients was also analyzed. Moreover, the fibulin-2 overexpression plasmid was constructed and transfected into human gastric cancer AGS and SGC-790 cell lines, so as to observe changes in β-catenin and its downstream indexes. Fibulin-2 messenger ribonucleic acid (mRNA) level in gastric cancer tissues was significantly lower than that in para-carcinoma tissues, while β-catenin mRNA level was significantly increased (p<0.05). The positive rate of fibulin-2 protein was 73.47% (36/49) in gastric cancer tissues and 16.33% (8/49) in para-carcinoma tissues, while that of β-catenin was 77.55% (38/49) in gastric cancer tissues and 28.57% (14/49) in para-carcinoma tissues, indicating that fibulin-2 protein is significantly deleted in gastric cancer tissues, and β-catenin protein is significantly upregulated (p<0.001). Fibulin-2 and β-catenin had a negative correlation (r=-0.361, p=0.003), but was closely correlated with the tumor size and lymph node metastasis (p<0.05). After overexpression of fibulin-2, expression of β-catenin, cyclin D1 and c-myc protein was obviously downregulated (p<0.05). The tumor suppressor gene, fibulin-2, is significantly deleted in gastric cancer tissues, while β-catenin is remarkably enriched. Overexpression of fibulin-2 can inhibit the development of gastric cancer by downregulating β-catenin.

Related Articles

Journal Cover

September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ma, H., Lian, C., & Song, Y. (2019). Fibulin-2 inhibits development of gastric cancer by downregulating β-catenin. Oncology Letters, 18, 2799-2804. https://doi.org/10.3892/ol.2019.10599
MLA
Ma, H., Lian, C., Song, Y."Fibulin-2 inhibits development of gastric cancer by downregulating β-catenin". Oncology Letters 18.3 (2019): 2799-2804.
Chicago
Ma, H., Lian, C., Song, Y."Fibulin-2 inhibits development of gastric cancer by downregulating β-catenin". Oncology Letters 18, no. 3 (2019): 2799-2804. https://doi.org/10.3892/ol.2019.10599