Open Access

Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells

  • Authors:
    • Ku‑Ke Ding
    • Fen Yang
    • Hui‑Qing Jiang
    • Zeng‑Qiang Yuan
    • Ling‑Ling Yin
    • Ling‑Yue Dong
    • Wei Cui
    • Qiao Gou
    • Xiao‑Dan Liu
    • Yu‑Mei Wu
    • Xiao‑Yan Jiang
    • Xin Zhang
    • Ping‑Kun Zhou
    • Chuan‑Jie Yang
  • View Affiliations

  • Published online on: July 9, 2019     https://doi.org/10.3892/ol.2019.10590
  • Pages: 2704-2711
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The effects of the immediate early response 5 (IER5) gene on the sensitivity of HeLa cells to radiation remain unclear. In the present study, stably transfected HeLa cells resulting in the knockdown or overexpression of IER5 were investigated. In addition, xenografts of normal, IER5‑silenced and ‑overexpressed HeLa cells were injected into nude mice and examined. The results demonstrated that the radiosensitivity of the IER5‑overexpressed HeLa cells was significantly increased compared with that of the normal and IER5‑silenced cells. The upregulation of IER5 effectively decreased cell proliferation and IER5 silencing promoted cell proliferation compared with that in the normal HeLa cells. Following irradiation of the cells with IER5 knockdown, cell cycle was arrested at the G2/M phase and an increase in the proportion of S phase cells was observed. By contrast, the overexpression of IER5 led to an increase in the proportion of G1 phase cells. Furthermore, the upregulation of IER5 inhibited tumor growth in vivo. The present findings demonstrate that the IER5 gene affects the radiosensitivity of HeLa cells and serves an important role in cell proliferation, suggesting that this gene may be a potential radiotherapeutic target in cervical cancer.
View Figures
View References

Related Articles

Journal Cover

September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L. ... Yang, C. (2019). Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells. Oncology Letters, 18, 2704-2711. https://doi.org/10.3892/ol.2019.10590
MLA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18.3 (2019): 2704-2711.
Chicago
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18, no. 3 (2019): 2704-2711. https://doi.org/10.3892/ol.2019.10590