Ursolic acid suppresses the biological function of osteosarcoma cells

  • Authors:
    • Yi Pei
    • Yueyan Zhang
    • Ke Zheng
    • Guanning Shang
    • Yuming Wang
    • Wei Wang
    • Enduo Qiu
    • Xiaojing Zhang
  • View Affiliations

  • Published online on: July 4, 2019     https://doi.org/10.3892/ol.2019.10561
  • Pages: 2628-2638
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Abstract

Osteosarcoma is a highly malignant tumour that occurs in adolescents. Upregulation or the constitutive activation of epidermal growth factor receptor (EGFR) is a hallmark of osteosarcoma. To investigate the effect of ursolic acid on the biological function of osteosarcoma, MTT assay was used to detect the effect of ursolic acid on the proliferation of HOS and MG63 cells, while flow cytometry was used to analyse the effect on the cell cycle and apoptosis. Transwell and Matrigel assays were used to detect the effect of ursolic acid on cell migration and invasion, respectively. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction were used to detect the effects of different concentrations of ursolic acid on EGFR signaling pathway‑related proteins, cell cycle, apoptosis and cell migration‑related proteins. After overexpression or silencing of EGFR, the effects of ursolic acid on EGFR pathway and cell biological function were subsequently detected, using the same methods. The present study identified that ursolic acid had inhibitory effects on the growth and metastatic ability of osteosarcoma cells by suppressing EGFR.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Pei, Y., Zhang, Y., Zheng, K., Shang, G., Wang, Y., Wang, W. ... Zhang, X. (2019). Ursolic acid suppresses the biological function of osteosarcoma cells. Oncology Letters, 18, 2628-2638. https://doi.org/10.3892/ol.2019.10561
MLA
Pei, Y., Zhang, Y., Zheng, K., Shang, G., Wang, Y., Wang, W., Qiu, E., Zhang, X."Ursolic acid suppresses the biological function of osteosarcoma cells". Oncology Letters 18.3 (2019): 2628-2638.
Chicago
Pei, Y., Zhang, Y., Zheng, K., Shang, G., Wang, Y., Wang, W., Qiu, E., Zhang, X."Ursolic acid suppresses the biological function of osteosarcoma cells". Oncology Letters 18, no. 3 (2019): 2628-2638. https://doi.org/10.3892/ol.2019.10561