miR‑296‑3p targets APEX1 to suppress cell migration and invasion of non‑small‑cell lung cancer

  • Authors:
    • Lifeng Wang
    • Ruilin Chen
    • Yongqing Zhang
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/ol.2019.10572
  • Pages: 2612-2618
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Abstract

Non‑small‑cell lung cancer (NSCLC) is the most common cause of cancer‑associated mortality worldwide. MicroRNAs (miRs) are a class of small non‑coding RNAs that are commonly dysregulated in human cancer. The aim of the current study was to evaluate the effect of miR‑296‑3p on the cell migration and invasion of NSCLC. Pairs of tumor tissues and para‑cancerous tissues (n=50) were collected from patients with NSCLC, and the expression of miR‑296‑3p was analyzed by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Additionally, tumor cell viability, migration and invasion were examined in vitro using Cell Counting Kit‑8, wound healing and Matrigel assays, respectively. Furthermore, potential targets of miR‑296‑3p were screened for using TargetScan and validated using a dual‑luciferase reporter assay. The expression levels of phosphoinositide‑3‑kinase (PI3K), AKT serine/threonine kinase (AKT), mammalian target of rapamycin (mTOR), matrix metallopeptidase 2 (MMP2) and SRY‑box 4 (SOX4) were detected by RT‑qPCR and western blot analysis. The data indicated that miR‑296‑3p was downregulated in tumor tissues compared with adjacent normal tissues. Overexpression of miR‑296‑3p inhibited NSCLC cell viability, migration and invasion in vitro. Furthermore, apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1) was identified as a direct target of miR‑296‑3p. APEX1 expression was upregulated in tumor tissues compared with para‑cancerous tissues, and the mRNA and protein expression levels of APEX1 were decreased following transfection of NSCLC cells with miR‑296‑3p mimics compared with control cells. Additional investigations revealed that miR‑296‑3p was involved in regulating the PI3K/AKT/mTOR signaling pathway, and miR‑296‑3p mimics decreased the mRNA and protein expression levels of MMP2 and SOX4. In summary, the findings demonstrated that miR‑296‑3p may function as a tumor suppressor, and inhibits the migration and invasion of NSCLC cells by targeting APEX1. miR‑296‑3p is therefore a potential therapeutic molecular modulator of NSCLC.
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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Wang, L., Chen, R., & Zhang, Y. (2019). miR‑296‑3p targets APEX1 to suppress cell migration and invasion of non‑small‑cell lung cancer. Oncology Letters, 18, 2612-2618. https://doi.org/10.3892/ol.2019.10572
MLA
Wang, L., Chen, R., Zhang, Y."miR‑296‑3p targets APEX1 to suppress cell migration and invasion of non‑small‑cell lung cancer". Oncology Letters 18.3 (2019): 2612-2618.
Chicago
Wang, L., Chen, R., Zhang, Y."miR‑296‑3p targets APEX1 to suppress cell migration and invasion of non‑small‑cell lung cancer". Oncology Letters 18, no. 3 (2019): 2612-2618. https://doi.org/10.3892/ol.2019.10572