Open Access

miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer

  • Authors:
    • Wei Zhao
    • Jianbao Zheng
    • Guangbing Wei
    • Kui Yang
    • Guanghui Wang
    • Xuejun Sun
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/ol.2019.10581
  • Pages: 2530-2536
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Colorectal cancer is a common gastrointestinal cancer ranking in third place of all cancers. Downregulation of miR‑148a has been observed in many tumors, and miR‑148a was found to be an oncogene in colorectal cancer. The aim of our study was to investigate the molecular mechanisms by which miR‑148a and ErbB3 proliferate and migrate in colorectal cancer. The expression of miR‑148a and ErbB3 were measured by western blot analysis and RT‑qPCR. MTT and transwell assays were performed to analyze the proliferative and migratory abilities. The dual luciferase reporter assay was employed to confirm miR‑148a regulated the expression of ErbB3 in colorectal cancer. It was discovered that miR‑148a was overexpressed while ErbB3 expression was low in colorectal cancer, and the mRNA level of miR‑148a had a negative correlation with the expression of ErbB3. Upregulation of miR‑148a suppressed the proliferation and migration in colorectal cancer cells. Furthermore, ErbB3 was identified as a direct target of miR‑148a, which suppressed the proliferation and migration through directly binding to the 3'UTR of ErbB3 mRNA. This study established that miR‑148a inhibited the proliferative and migratory abilities through mediating the expression of ErbB3. The newly identified miR‑148a/ErbB3 axis provides novel insight into the pathogenesis of colorectal cancer, and represents a potential target for treatment of colorectal cancer.

Related Articles

Journal Cover

September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhao, W., Zheng, J., Wei, G., Yang, K., Wang, G., & Sun, X. (2019). miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer. Oncology Letters, 18, 2530-2536. https://doi.org/10.3892/ol.2019.10581
MLA
Zhao, W., Zheng, J., Wei, G., Yang, K., Wang, G., Sun, X."miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer". Oncology Letters 18.3 (2019): 2530-2536.
Chicago
Zhao, W., Zheng, J., Wei, G., Yang, K., Wang, G., Sun, X."miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer". Oncology Letters 18, no. 3 (2019): 2530-2536. https://doi.org/10.3892/ol.2019.10581