Open Access

MicroRNA-744 promotes carcinogenesis in osteosarcoma through targeting LATS2

  • Authors:
    • Liangzhi Sun
    • Ming Liu
    • Suxian Luan
    • Yulin Shi
    • Qiang Wang
  • View Affiliations

  • Published online on: June 26, 2019     https://doi.org/10.3892/ol.2019.10530
  • Pages: 2523-2529
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma (OS) mortality rate is increasing. Various microRNAs (miRNAs) have been investigated in the pathological process of OS except for miR-744. Hence, this research was designed to explore miR-744 function in OS. RT-qPCR and western blot analysis were used to quantify miR-744 and large tumor suppressor kinase 2 (LATS2) expression levels. The function of miR-744 was investigated using MTT and Transwell assays. Target gene of miR-744 was verified by dual-luciferase reporter assay. miR-744 expression was increased in OS, which was associated with worse clinical features and prognosis of OS patients. Importantly, miR-744 promoted cell viability and metastasis in OS. Furthermore, miR-744 induced Wnt/β-catenin pathway and epithelial‑mesenchymal transition (EMT) in OS. In addition, miR-744 directly targeted LATS2 and blocked its expression in OS. Moreover, upregulation of LATS2 weakened the promotion of cell viability and metastasis induced by miR-744 in OS. In conclusion, miR-744 accelerated OS progression through restraining LATS2 and activating Wnt/β‑catenin pathway and EMT.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Sun, L., Liu, M., Luan, S., Shi, Y., & Wang, Q. (2019). MicroRNA-744 promotes carcinogenesis in osteosarcoma through targeting LATS2. Oncology Letters, 18, 2523-2529. https://doi.org/10.3892/ol.2019.10530
MLA
Sun, L., Liu, M., Luan, S., Shi, Y., Wang, Q."MicroRNA-744 promotes carcinogenesis in osteosarcoma through targeting LATS2". Oncology Letters 18.3 (2019): 2523-2529.
Chicago
Sun, L., Liu, M., Luan, S., Shi, Y., Wang, Q."MicroRNA-744 promotes carcinogenesis in osteosarcoma through targeting LATS2". Oncology Letters 18, no. 3 (2019): 2523-2529. https://doi.org/10.3892/ol.2019.10530