Open Access

Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1

  • Authors:
    • Zhitian Shi
    • Dong Wei
    • Huamei Wu
    • Jiayun Ge
    • Xuefen Lei
    • Zhitang Guo
    • Renchao Zou
    • Shufeng Xiao
    • Tiangen Wu
    • Ruicheng Ma
    • Runyao Ai
    • Lin Wang
  • View Affiliations

  • Published online on: April 16, 2019     https://doi.org/10.3892/ol.2019.10258
  • Pages: 5565-5571
  • Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

It was previously demonstrated that the long non‑coding RNA (lncRNA) small NF90‑associated RNA (snaR) served an oncogenic role in human colon cancer, although its roles in other types of cancer remain unknown. To investigate the potential involvement of lncRNA snaR in hepatocellular carcinoma (HCC), expression of snaR in liver biopsies and plasma of patients with HCC and healthy controls was detected by reverse transcription‑quantitative polymerase chain reaction. ELISA was used to determine the protein expression levels of transforming growth factor‑β1 (TGF‑β1). A snaR expression vector was transfected into HCC cells, and the effects on cell migration and invasion were analyzed by Transwell migration and Matrigel invasion assays, respectively. The protein expression levels of TGF‑β1 in HCC cells were detected by western blotting. The expression of snaR and TGF‑β1 was significantly increased in the patients with HCC compared with the healthy controls. The plasma expression levels of snaR and TGF‑β1 were positively correlated in patients with HCC; however, not in healthy controls. snaR overexpression significantly promoted cancer cell migration and invasion, and additionally increased TGF‑β1 expression. Treatment with TGF‑β1 did not significantly affect snaR expression. A TGF‑β1 inhibitor attenuated the effects of snaR overexpression in cancer cell migration and invasion. snaR may promote the metastasis of liver cancer through TGF‑β1.

References

1 

Chang SS, Bochner BH, Chou R, Dreicer R, Kamat AM, Lerner SP, Lotan Y, Meeks JJ, Michalski JM, Morgan TM, et al: Treatment of non-metastatic muscle-invasive bladder cancer: AUA/ASCO/ASTRO/SUO guideline. J Urol. 198:552–559. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Khan MS, Kirkwood AA, Tsigani T, Lowe H, Goldstein R, Hartley JA, Caplin ME and Meyer T: Early changes in circulating tumor cells are associated with response and survival following treatment of metastatic neuroendocrine neoplasms. Clin Cancer Res. 22:79–85. 2016. View Article : Google Scholar : PubMed/NCBI

3 

White DL, Kanwal F, Jiao L and El Serag HB: Epidemiology of hepatocellular carcinoma. Hepatocellular Carcinoma Diagnosis and Treatment. Carr BI: Springer; Cham, Switzerland: pp. 3–24. 2016, View Article : Google Scholar

4 

Kummar S and Shafi NQ: Metastatic hepatocellular carcinoma. Clin Oncol (R Coll Radiol). 15:288–294. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Zhu RX, Seto WK, Lai CL and Yuen MF: Epidemiology of hepatocellular carcinoma in the Asia-Pacific region. Gut Liver. 10:332–339. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Derynck R, Akhurst RJ and Balmain A: TGF-beta signaling in tumor suppression and cancer progression. Nat Genet. 29:117–129. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Katsuno Y, Lamouille S and Derynck R: TGF-β signaling and epithelial-mesenchymal transition in cancer progression. Curr Opin Oncol. 25:76–84. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Wei W and Birrer MJ: Abstract 5401: TGF-β signaling inhibition as a potential approach to target suboptimally debulked ovarian tumors. Cancer Res. 75:5401. 2015. View Article : Google Scholar

9 

Yuan J, Yang F, Wang F, Ma JZ, Guo YJ, Tao QF, Liu F, Pan W, Wang TT, Zhou CC, et al: A long noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma. Cancer Cell. 25:666–681. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Li W and Kang Y: A new Lnc in metastasis: Long noncoding RNA mediates the prometastatic functions of TGF-β. Cancer Cell. 25:557–559. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: Insights into functions. Nat Rev Genet. 10:155–159. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Gutschner T and Diederichs S: The hallmarks of cancer: A long non-coding RNA point of view. RNA Biol. 9:703–719. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Lee H, Kim C, Ku JL, Kim W, Yoon SK, Kuh HJ, Lee JH, Nam SW and Lee EK: A long non-coding RNA snaR contributes to 5-fluorouracil resistance in human colon cancer cells. Mol Cells. 37:540–546. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

El-Serag HB: Epidemiology of viral hepatitis and hepatocellular carcinoma. Gastroenterology. 142:1264–1273.e1. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Akhurst RJ and Derynck R: TGF-beta signaling in cancer-a double-edged sword. Trends Cell Biol. 11:S44–S51. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Markowitz SD and Roberts AB: Tumor suppressor activity of the TGF-beta pathway in human cancers. Cytokine Growth Factor Rev. 7:93–102. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Pardali K and Moustakas A: Actions of TGF-beta as tumor suppressor and pro-metastatic factor in human cancer. Biochim Biophys Acta. 1775:21–62. 2007.PubMed/NCBI

19 

Huang JF, Guo YJ, Zhao CX, Yuan SX, Wang Y, Tang GN, Zhou WP and Sun SH: Hepatitis B virus X protein (HBx)-related long noncoding RNA (lncRNA) down-regulated expression by HBx (Dreh) inhibits hepatocellular carcinoma metastasis by targeting the intermediate filament protein vimentin. Hepatology. 57:1882–1892. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Yang X, Xie X, Xiao YF, Xie R, Hu CJ, Tang B, Li BS and Yang SM: The emergence of long non-coding RNAs in the tumorigenesis of hepatocellular carcinoma. Cancer Lett. 360:119–124. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Chen Z, Yu C, Zhan L, Pan Y, Chen L and Sun C: LncRNA CRNDE promotes hepatic carcinoma cell proliferation, migration and invasion by suppressing miR-384. Am J Cancer Res. 6:2299–2309. 2016.PubMed/NCBI

Related Articles

Journal Cover

June 2019
Volume 17 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Shi, Z., Wei, D., Wu, H., Ge, J., Lei, X., Guo, Z. ... Wang, L. (2019). Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1. Oncology Letters, 17, 5565-5571. https://doi.org/10.3892/ol.2019.10258
MLA
Shi, Z., Wei, D., Wu, H., Ge, J., Lei, X., Guo, Z., Zou, R., Xiao, S., Wu, T., Ma, R., Ai, R., Wang, L."Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1". Oncology Letters 17.6 (2019): 5565-5571.
Chicago
Shi, Z., Wei, D., Wu, H., Ge, J., Lei, X., Guo, Z., Zou, R., Xiao, S., Wu, T., Ma, R., Ai, R., Wang, L."Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1". Oncology Letters 17, no. 6 (2019): 5565-5571. https://doi.org/10.3892/ol.2019.10258