Open Access

miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1

  • Authors:
    • Changming Tao
    • Huiling Sun
    • Weiwei Sang
    • Shanshan Li
  • View Affiliations

  • Published online on: March 29, 2019     https://doi.org/10.3892/ol.2019.10199
  • Pages: 5108-5114
  • Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Liver cancer is a malignant tumor that threatens human health worldwide. It has poor prognosis rates and ineffective therapeutic options. Recently, various miRNAs have been proven to exert promoting or inhibiting functions in different malignancies. However, the definitive mechanisms of miR-99a in liver cancer remain unclear. In the current study, we explored the relationships between the expression of miR-99a and HOXA1 in liver cancer tissues and cells to explore their combined effects on the occurrence and metastasis of liver cancer. The expression of miR-99a and HOXA1 in liver cancer tissue samples and cells was measured by RT-qPCR. Following transfection, transwell assays were conducted to assess the invasion and migration capacities of liver cancer cells. Subsequently, western blots and luciferase reporter assays were performed in liver cancer cells to identify the target of miR-99a. The data indicated that miRNA-99a expression was significantly reduced in both liver cancer tissue samples and cells compared with normal tissues and normal liver cells respectively. By contrast, the HOXA1 expression levels in liver cancer tissues and cells were significantly increased in contrast to the control group. The findings also revealed that the miR-99a expression was negatively correlated with HOXA1 expression in liver cancer tissue samples and miR-99a could suppress cell invasion and migration by targeting HOXA1 in liver cancer.

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June 2019
Volume 17 Issue 6

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Copy and paste a formatted citation
APA
Tao, C., Sun, H., Sang, W., & Li, S. (2019). miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1. Oncology Letters, 17, 5108-5114. https://doi.org/10.3892/ol.2019.10199
MLA
Tao, C., Sun, H., Sang, W., Li, S."miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1". Oncology Letters 17.6 (2019): 5108-5114.
Chicago
Tao, C., Sun, H., Sang, W., Li, S."miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1". Oncology Letters 17, no. 6 (2019): 5108-5114. https://doi.org/10.3892/ol.2019.10199