Open Access

Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells

  • Authors:
    • Tongshan Wang
    • Jun Liu
    • Suihui Li
    • Zhengang Yuan
    • Xiangzhong Huang
  • View Affiliations

  • Published online on: April 1, 2019     https://doi.org/10.3892/ol.2019.10201
  • Pages: 5103-5107
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of long intergenic non‑coding RNA (lincRNA)‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells were assessed to explore the underlying mechanism. The lincRNA‑p21 small interfering RNA (siRNA) lentivirus vector was constructed, transfected and screened to obtain a stable cell line, which constituted the experimental group. At the same time, the empty virus vector was transfected as the control group. The messenger RNA (mRNA) expression of lincRNA‑p21 in cells was detected via reverse transcription‑polymerase chain reaction (RT‑PCR). The proliferation ability of cells was detected via Cell Counting kit‑8 (CCK‑8) assay. Transwell chamber experiment was used to observe cell migration and invasion ability. Compared with that in the control group, the mRNA expression level of lincRNA‑p21 in cells in the experimental group was obviously decreased (p<0.05). Results of CCK‑8 showed that the proliferation ability of liver cancer cells was remarkably higher than that in the control group after knockout of lincRNA‑p21 (p<0.05). Results of the Transwell chamber experiment revealed that the invasion and migration ability of HepG2 cells in experimental group was markedly higher than that in control group (p<0.05). When lincRNA‑p21 was inhibited, the proliferation, invasion and migration ability of HepG2 cells were significantly enhanced, and the apoptosis rate was significantly decreased. Thus, lincRNA‑p21 on the surface may play an inhibitory role in the occurrence, development and metastasis of liver cancer.

References

1 

Sun T, Liu H and Ming L: Multiple roles of autophagy in the sorafenib resistance of hepatocellular carcinoma. Cell Physiol Biochem. 44:716–727. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Pinter M, Weinmann A, Wörns MA, Hucke F, Bota S, Marquardt JU, Duda DG, Jain RK, Galle PR, Trauner M, et al: Use of inhibitors of the renin-angiotensin system is associated with longer survival in patients with hepatocellular carcinoma. United European Gastroenterol J. 5:987–996. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Sanduzzi Zamparelli M, Rocco A, Compare D and Nardone G: The gut microbiota: A new potential driving force in liver cirrhosis and hepatocellular carcinoma. United European Gastroenterol J. 5:944–953. 2017. View Article : Google Scholar : PubMed/NCBI

4 

An C, Hu ZL, Liang P, Cheng ZG, Han ZY, Yu J and Liu FY: Ultrasound-guided percutaneous microwave ablation vs. surgical resection for thoracoabdominal wall implants from hepatocellular carcinoma: Intermediate-term results. Int J Hyperthermia. 21:1–10. 2017.

5 

Chen X, Jiang W, Yue C, Zhang W, Tong C, Dai D, Cheng B, Huang C and Lu L: Heparanase contributes to trans-endothelial migration of hepatocellular carcinoma cells. J Cancer. 8:3309–3317. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Chen S, Liang H, Yang H, Zhou K, Xu L, Liu J, Lai B, Song L, Luo H, Peng J, et al: LincRNa-p21: Function and mechanism in cancer. Med Oncol. 34:982017. View Article : Google Scholar : PubMed/NCBI

7 

Chillón I and Pyle AM: Inverted repeat Alu elements in the human lincRNA-p21 adopt a conserved secondary structure that regulates RNA function. Nucleic Acids Res. 44:9462–9471. 2016.PubMed/NCBI

8 

Shen Y, Liu Y, Sun T and Yang W: LincRNA-p21 knockdown enhances radiosensitivity of hypoxic tumor cells by reducing autophagy through HIF-1/Akt/mTOR/P70S6K pathway. Exp Cell Res. 358:188–198. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Chen Y, Wei G, Xia H, Yu H, Tang Q and Bi F: Down regulation of lincRNA-p21 contributes to gastric cancer development through Hippo-independent activation of YAP. Oncotarget. 8:63813–63824. 2017.PubMed/NCBI

10 

Wang JY, Fang M, Boye A, Wu C, Wu JJ, Ma Y, Hou S, Kan Y and Yang Y: Interaction of microRNA-21/145 and Smad3 domain-specific phosphorylation in hepatocellular carcinoma. Oncotarget. 8:84958–84973. 2017.PubMed/NCBI

11 

Feng LH, Wang H, Dong H, Zhu YY and Cong WM: The stromal morphological changes for differential diagnosis of uninodular high-grade dysplastic nodule and well-differentiated small hepatocellular carcinoma. Oncotarget. 8:87329–87339. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Ye JZ, Chen JZ, Li ZH, Bai T, Chen J, Zhu SL, Li LQ and Wu FX: Efficacy of postoperative adjuvant transcatheter arterial chemoembolization in hepatocellular carcinoma patients with microvascular invasion. World J Gastroenterol. 23:7415–7424. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Sim HW and Knox J: Hepatocellular carcinoma in the era of immunotherapy. Curr Probl Cancer. 42:40–48. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Honda H, Takamura M, Yamagiwa S, Genda T, Horigome R, Kimura N, Setsu T, Tominaga K, Kamimura H, Matsuda Y, et al: Overexpression of a disintegrin and metalloproteinase 21 is associated with motility, metastasis, and poor prognosis in hepatocellular carcinoma. Sci Rep. 7:154852017. View Article : Google Scholar : PubMed/NCBI

15 

Ding G, Peng Z, Shang J, Kang Y, Ning H and Mao C: LincRNA-p21 inhibits invasion and metastasis of hepatocellular carcinoma through miR-9/E-cadherin cascade signaling pathway molecular mechanism. OncoTargets Ther. 10:3241–3247. 2017. View Article : Google Scholar

16 

Jia M, Jiang L, Wang YD, Huang JZ, Yu M and Xue HZ: lincRNA-p21 inhibits invasion and metastasis of hepatocellular carcinoma through Notch signaling-induced epithelial-mesenchymal transition. Hepatol Res. 46:1137–1144. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Yu F, Guo Y, Chen B, Shi L, Dong P, Zhou M and Zheng J: LincRNA-p21 Inhibits the Wnt/β-catenin pathway in activated hepatic stellate cells via sponging microRNA-17-5p. Cell Physiol Biochem. 41:1970–1980. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Castellano JJ, Navarro A, Viñolas N, Marrades RM, Moises J, Cordeiro A, Saco A, Muñoz C, Fuster D, Molins L, et al: LincRNA-p21 impacts prognosis in resected non-small cell lung cancer patients through angiogenesis regulation. J Thorac Oncol. 11:2173–2182. 2016. View Article : Google Scholar : PubMed/NCBI

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June 2019
Volume 17 Issue 6

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Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Wang, T., Liu, J., Li, S., Yuan, Z., & Huang, X. (2019). Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells. Oncology Letters, 17, 5103-5107. https://doi.org/10.3892/ol.2019.10201
MLA
Wang, T., Liu, J., Li, S., Yuan, Z., Huang, X."Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells". Oncology Letters 17.6 (2019): 5103-5107.
Chicago
Wang, T., Liu, J., Li, S., Yuan, Z., Huang, X."Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells". Oncology Letters 17, no. 6 (2019): 5103-5107. https://doi.org/10.3892/ol.2019.10201