Open Access

Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells

  • Authors:
    • Tongshan Wang
    • Jun Liu
    • Suihui Li
    • Zhengang Yuan
    • Xiangzhong Huang
  • View Affiliations

  • Published online on: April 1, 2019     https://doi.org/10.3892/ol.2019.10201
  • Pages: 5103-5107
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Effects of long intergenic non‑coding RNA (lincRNA)‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells were assessed to explore the underlying mechanism. The lincRNA‑p21 small interfering RNA (siRNA) lentivirus vector was constructed, transfected and screened to obtain a stable cell line, which constituted the experimental group. At the same time, the empty virus vector was transfected as the control group. The messenger RNA (mRNA) expression of lincRNA‑p21 in cells was detected via reverse transcription‑polymerase chain reaction (RT‑PCR). The proliferation ability of cells was detected via Cell Counting kit‑8 (CCK‑8) assay. Transwell chamber experiment was used to observe cell migration and invasion ability. Compared with that in the control group, the mRNA expression level of lincRNA‑p21 in cells in the experimental group was obviously decreased (p<0.05). Results of CCK‑8 showed that the proliferation ability of liver cancer cells was remarkably higher than that in the control group after knockout of lincRNA‑p21 (p<0.05). Results of the Transwell chamber experiment revealed that the invasion and migration ability of HepG2 cells in experimental group was markedly higher than that in control group (p<0.05). When lincRNA‑p21 was inhibited, the proliferation, invasion and migration ability of HepG2 cells were significantly enhanced, and the apoptosis rate was significantly decreased. Thus, lincRNA‑p21 on the surface may play an inhibitory role in the occurrence, development and metastasis of liver cancer.
View Figures
View References

Related Articles

Journal Cover

June-2019
Volume 17 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Wang, T., Liu, J., Li, S., Yuan, Z., & Huang, X. (2019). Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells. Oncology Letters, 17, 5103-5107. https://doi.org/10.3892/ol.2019.10201
MLA
Wang, T., Liu, J., Li, S., Yuan, Z., Huang, X."Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells". Oncology Letters 17.6 (2019): 5103-5107.
Chicago
Wang, T., Liu, J., Li, S., Yuan, Z., Huang, X."Effects of knockout of lincRNA‑p21 on the proliferation, migration and invasion ability of HepG2 liver cancer cells". Oncology Letters 17, no. 6 (2019): 5103-5107. https://doi.org/10.3892/ol.2019.10201