Open Access

miR‑185‑5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma

  • Authors:
    • Yuexiang Niu
    • Gongen Tang
  • View Affiliations

  • Published online on: March 13, 2019     https://doi.org/10.3892/ol.2019.10144
  • Pages: 5087-5093
  • Copyright: © Niu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies demonstrated microRNA‑185 (miR‑185) as a tumor suppressive microRNA (miRNA) in various types of cancer. The current study aimed to verify this finding in hepatocellular carcinoma (HCC) and explored the downstream channel of miR‑185‑5p. We detected miR‑185‑5p and Rho‑associated coiled‑coil containing protein kinase 2 (ROCK2) mRNA and protein levels by reverse transcription-quantitative PCR (RT‑qPCR) and western blotting in HCC tissues and cell lines. Luciferase reporter assay proved the direct relationship between miR‑185‑5p and ROCK2. Cell migration and invasion were assessed via Transwell assay. miR‑185‑5p level was reduced in HCC tissues and cell lines. miR‑185‑5p overexpression impeded migration and invasion of HCC cells. Moreover, miR‑185‑5p directly targeted ROCK2 which was repressed by miR‑185‑5p in HCC. In addition, ROCK2 contributed to cell metastasis of HCC. In summary, miR‑185‑5p inhibited cell metastasis of HCC by suppressing ROCK2. The novel miR‑185/ROCK2 axis shows potential in improving the therapies of HCC and enhancing HCC survival.

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June 2019
Volume 17 Issue 6

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Copy and paste a formatted citation
APA
Niu, Y., & Niu, Y. (2019). miR‑185‑5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma. Oncology Letters, 17, 5087-5093. https://doi.org/10.3892/ol.2019.10144
MLA
Niu, Y., Tang, G."miR‑185‑5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma". Oncology Letters 17.6 (2019): 5087-5093.
Chicago
Niu, Y., Tang, G."miR‑185‑5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma". Oncology Letters 17, no. 6 (2019): 5087-5093. https://doi.org/10.3892/ol.2019.10144