miR‑185‑5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma
Affiliations: Department of Infectious Diseases, Linyi Central Hospital, Linyi, Shandong 276400, P.R. China
- Published online on: March 13, 2019 https://doi.org/10.3892/ol.2019.10144
Copyright: © Niu
et al. This is an open access article distributed under the
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Previous studies demonstrated microRNA‑185 (miR‑185) as a tumor suppressive microRNA (miRNA) in various types of cancer. The current study aimed to verify this finding in hepatocellular carcinoma (HCC) and explored the downstream channel of miR‑185‑5p. We detected miR‑185‑5p and Rho‑associated coiled‑coil containing protein kinase 2 (ROCK2) mRNA and protein levels by reverse transcription-quantitative PCR (RT‑qPCR) and western blotting in HCC tissues and cell lines. Luciferase reporter assay proved the direct relationship between miR‑185‑5p and ROCK2. Cell migration and invasion were assessed via Transwell assay. miR‑185‑5p level was reduced in HCC tissues and cell lines. miR‑185‑5p overexpression impeded migration and invasion of HCC cells. Moreover, miR‑185‑5p directly targeted ROCK2 which was repressed by miR‑185‑5p in HCC. In addition, ROCK2 contributed to cell metastasis of HCC. In summary, miR‑185‑5p inhibited cell metastasis of HCC by suppressing ROCK2. The novel miR‑185/ROCK2 axis shows potential in improving the therapies of HCC and enhancing HCC survival.