Open Access

Identification of a 13‑gene‑based classifier as a potential biomarker to predict the effects of fluorouracil‑based chemotherapy in colorectal cancer

  • Authors:
    • Zuhuan Gan
    • Qiyuan Zou
    • Yan Lin
    • Zihai Xu
    • Zhong Huang
    • Zhichao Chen
    • Yufeng Lv
  • View Affiliations

  • Published online on: March 19, 2019     https://doi.org/10.3892/ol.2019.10159
  • Pages: 5057-5063
  • Copyright: © Gan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the current study was to develop a predictor classifier for response to fluorouracil‑based chemotherapy in patients with advanced colorectal cancer (CRC) using microarray gene expression profiles of primary CRC tissues. Using two expression profiles downloaded from the Gene Expression Omnibus database, differentially expressed genes (DEGs) between responders and non‑responders to fluorouracil‑based chemotherapy were identified. A total of 791 DEGs, including 303 that were upregulated and 488 that were downregulated in responders, were identified. Functional enrichment analysis revealed that the DEGs were primarily involved in ‘cell mitosis’, ‘DNA replication’ and ‘cell cycle’ signaling pathways. Following feature selection using two methods, a random forest classifier for response to fluorouracil‑based chemotherapy with 13 DEGs was constructed. The accuracy of the 13‑gene classifier was 0.930 in the training set and 0.810 in the validation set. The receiver operating characteristic curve analysis revealed that the area under the curve was 1.000 in the training set and 0.873 in the validation set (P=0.227). The 13‑gene‑based classifier described in the current study may be used as a potential biomarker to predict the effects of fluorouracil‑based chemotherapy in patients with CRC.
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June 2019
Volume 17 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Gan, Z., Zou, Q., Lin, Y., Xu, Z., Huang, Z., Chen, Z., & Lv, Y. (2019). Identification of a 13‑gene‑based classifier as a potential biomarker to predict the effects of fluorouracil‑based chemotherapy in colorectal cancer. Oncology Letters, 17, 5057-5063. https://doi.org/10.3892/ol.2019.10159
MLA
Gan, Z., Zou, Q., Lin, Y., Xu, Z., Huang, Z., Chen, Z., Lv, Y."Identification of a 13‑gene‑based classifier as a potential biomarker to predict the effects of fluorouracil‑based chemotherapy in colorectal cancer". Oncology Letters 17.6 (2019): 5057-5063.
Chicago
Gan, Z., Zou, Q., Lin, Y., Xu, Z., Huang, Z., Chen, Z., Lv, Y."Identification of a 13‑gene‑based classifier as a potential biomarker to predict the effects of fluorouracil‑based chemotherapy in colorectal cancer". Oncology Letters 17, no. 6 (2019): 5057-5063. https://doi.org/10.3892/ol.2019.10159