Open Access

Analysis of the role of DAMTC in lung adenocarcinoma cells based on the DNA microarrays

  • Authors:
    • Binliang Wang
    • Yuanyuan Cai
    • Yiming Kong
    • Xiaobo Li
    • Haiwei Fu
    • Song Zhang
    • Tianwei Zhang
  • View Affiliations

  • Published online on: March 15, 2019     https://doi.org/10.3892/ol.2019.10146
  • Pages: 4787-4794
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effect of 7, 8‑diacetoxy‑4‑methylcoumarin (DAMTC) on lung adenocarcinoma cells (A549) and analyze the molecular mechanism underlying DAMTC‑treated lung adenocarcinoma. Gene expression profile GSE29698 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) in 3 DAMTC‑treated A549 samples were analyzed and compared with 3 DAMTC‑untreated samples using the limma package. Gene Ontology (GO) and pathway enrichment analyses of DEGs were performed, followed by the functional annotation and protein‑protein interaction (PPI) network construction. Finally, pathway crosstalk analysis was conducted. A total of 500 upregulated and 389 downregulated DEGs were identified. The upregulated and downregulated DEGs were enriched in different GO terms and pathways, including metabolic process, p53 signaling pathway and metabolic pathways. A total of 9 DEGs were determined to have node degrees >16 in the PPI network, including interleukin 6 (IL6), MDM2 oncogene, E3 ubiquitin protein ligase (MDM2), cell division cycle 42 (CDC42) and MYC associated factor X (MAX). Furthermore, numerous DEGs were identified to function as transcription factors and tumor suppressor genes, including histone deacetylase 3 and MAX. Additionally, apoptosis, tight junction, and endocytosis pathway were determined to cross‑talk with small cell and non‑small cell lung cancer. The DEGs (IL6, MDM2, CDC42 and MAX) involved in different pathways, including the p53 signaling pathway and endocytosis, may be the potential targets for DAMTC in lung adenocarcinoma. The elucidation of the underlying mechanism of the DAMTC effect may make it a potential drug.

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June 2019
Volume 17 Issue 6

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Copy and paste a formatted citation
APA
Wang, B., Cai, Y., Kong, Y., Li, X., Fu, H., Zhang, S., & Zhang, T. (2019). Analysis of the role of DAMTC in lung adenocarcinoma cells based on the DNA microarrays. Oncology Letters, 17, 4787-4794. https://doi.org/10.3892/ol.2019.10146
MLA
Wang, B., Cai, Y., Kong, Y., Li, X., Fu, H., Zhang, S., Zhang, T."Analysis of the role of DAMTC in lung adenocarcinoma cells based on the DNA microarrays". Oncology Letters 17.6 (2019): 4787-4794.
Chicago
Wang, B., Cai, Y., Kong, Y., Li, X., Fu, H., Zhang, S., Zhang, T."Analysis of the role of DAMTC in lung adenocarcinoma cells based on the DNA microarrays". Oncology Letters 17, no. 6 (2019): 4787-4794. https://doi.org/10.3892/ol.2019.10146