Open Access

Glycylglycine plays critical roles in the proliferation of spermatogonial stem cells

  • Authors:
    • Bo Xu
    • Xiang Wei
    • Minjian Chen
    • Kaipeng Xie
    • Yuqing Zhang
    • Zhenyao Huang
    • Tianyu Dong
    • Weiyue Hu
    • Kun Zhou
    • Xiumei Han
    • Xin Wu
    • Yankai Xia
  • View Affiliations

  • Published online on: August 23, 2019     https://doi.org/10.3892/mmr.2019.10609
  • Pages: 3802-3810
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glial cell line‑derived neurotrophic factor (GDNF) is critical for the proliferation of spermatogonial stem cells (SSCs), but the underlying mechanisms remain poorly understood. In this study, an unbiased metabolomic analysis was performed to examine the metabolic modifications in SSCs following GDNF deprivation, and 11 metabolites were observed to decrease while three increased. Of the 11 decreased metabolites identified, glycylglycine was observed to significantly rescue the proliferation of the impaired SSCs, while no such effect was observed by adding sorbitol. However, the expression of self‑renewal genes, including B‑cell CLL/lymphoma 6 member B, ETS variant 5, GDNF family receptor α1 and early growth response protein 4 remained unaltered following glycylglycine treatment. This finding suggests that although glycylglycine serves an important role in the proliferation of SSCs, it is not required for the self‑renewal of SSCs.

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October 2019
Volume 20 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
APA
Xu, B., Wei, X., Chen, M., Xie, K., Zhang, Y., Huang, Z. ... Xia, Y. (2019). Glycylglycine plays critical roles in the proliferation of spermatogonial stem cells. Molecular Medicine Reports, 20, 3802-3810. https://doi.org/10.3892/mmr.2019.10609
MLA
Xu, B., Wei, X., Chen, M., Xie, K., Zhang, Y., Huang, Z., Dong, T., Hu, W., Zhou, K., Han, X., Wu, X., Xia, Y."Glycylglycine plays critical roles in the proliferation of spermatogonial stem cells". Molecular Medicine Reports 20.4 (2019): 3802-3810.
Chicago
Xu, B., Wei, X., Chen, M., Xie, K., Zhang, Y., Huang, Z., Dong, T., Hu, W., Zhou, K., Han, X., Wu, X., Xia, Y."Glycylglycine plays critical roles in the proliferation of spermatogonial stem cells". Molecular Medicine Reports 20, no. 4 (2019): 3802-3810. https://doi.org/10.3892/mmr.2019.10609