Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis

  • Authors:
    • Lei‑Lei Gao
    • Fei Xu
    • Zhen Jin
    • Xiao‑Yan Ying
    • Jin‑Wei Liu
  • View Affiliations

  • Published online on: August 22, 2019     https://doi.org/10.3892/mmr.2019.10605
  • Pages: 3573-3582
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Abstract

Microtubule‑severing proteins (MTSPs) are a group of microtubule‑associated proteins essential for multiple microtubule‑related processes, including mitosis and meiosis. Katanin p60 ATPase‑containing subunit A‑like 1 (p60 katanin‑like 1) is an MTSP that maintains the density of spindle microtubules at the poles in mitotic cells; however, to date, there have been no studies about its role in female meiosis. Using in vitro‑matured (IVM) oocytes as a model, it was first revealed that p60 katanin‑like 1 was predominant in the ovaries and oocytes, indicating its essential roles in oocyte meiosis. It was also revealed that p60 katanin‑like 1 was concentrated at the spindle poles and co‑localized and interacted with γ‑tubulin, indicating that it may be involved in pole organization. Next, specific siRNA was used to deplete p60 katanin‑like 1; the spindle organization was severely disrupted and characterized by an abnormal width:length ratio, multipolarity and extra aster microtubules out of the main spindles. Finally, it was determined that p60 katanin‑like 1 knockdown retarded oocyte meiosis, reduced fertilization, and caused abnormal mitochondrial distribution. Collectively, these results indicated that p60 katanin‑like 1 is essential for oocyte meiosis by ensuring the integrity of the spindle poles.

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October 2019
Volume 20 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Gao, L., Xu, F., Jin, Z., Ying, X., & Liu, J. (2019). Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis. Molecular Medicine Reports, 20, 3573-3582. https://doi.org/10.3892/mmr.2019.10605
MLA
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20.4 (2019): 3573-3582.
Chicago
Gao, L., Xu, F., Jin, Z., Ying, X., Liu, J."Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosis". Molecular Medicine Reports 20, no. 4 (2019): 3573-3582. https://doi.org/10.3892/mmr.2019.10605