Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells

  • Authors:
    • Jiajun Chen
    • Yue Zhou
    • Xiangyu Zhou
    • Jia Li
    • Feng Liu
    • Qingwei Zhou
  • View Affiliations

  • Published online on: July 3, 2019     https://doi.org/10.3892/mmr.2019.10459
  • Pages: 2389-2395
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Abstract

The current study investigated the effect of plasmid small interfering RNA (psiRNA)‑mediated silencing of signal transducer and activator of transcription‑3 (STAT3) on the invasion, apoptosis, and expression levels of cyclin D1, caspase‑3 and B‑cell lymphoma‑2 (Bcl‑2) in C6 glioma cells. Cell invasion was determined using a Transwell assay, while the apoptosis rate and cell cycle distribution of cells were assessed using Annexin V‑FITC/PI double staining. The expression levels of cyclin D1, caspase‑3 and Bcl‑2 proteins were measured by western blotting. Transfection with psiRNA‑STAT3 was observed to significantly decrease the number of transmembrane cells compared with the control groups (P<0.05). In addition, the proportion of cells at G0/G1 phase was increased in the psiRNA‑STAT3 group compared with the controls. Western blotting indicated that psiRNA‑STAT3 decreased the expression of cyclin D1, caspase‑3 and Bcl‑2 proteins. Taken together, psiRNA‑STAT3 inhibited the migration and invasive abilities, and induced the apoptosis of C6 glioma cells, possibly through regulation of the expression of cyclin D1, caspase‑3 and Bcl‑2 proteins.

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September 2019
Volume 20 Issue 3

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Online ISSN:1791-3004

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APA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., & Zhou, Q. (2019). Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells. Molecular Medicine Reports, 20, 2389-2395. https://doi.org/10.3892/mmr.2019.10459
MLA
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20.3 (2019): 2389-2395.
Chicago
Chen, J., Zhou, Y., Zhou, X., Li, J., Liu, F., Zhou, Q."Inhibitory effects of plasmid small interfering RNA targeting signal transducer and activator of transcription‑3 in C6 glioma cells". Molecular Medicine Reports 20, no. 3 (2019): 2389-2395. https://doi.org/10.3892/mmr.2019.10459