Open Access

MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin

  • Authors:
    • Hao Wang
    • Lei Liu
    • Shiyuan Fang
  • View Affiliations

  • Published online on: July 1, 2019     https://doi.org/10.3892/mmr.2019.10447
  • Pages: 2236-2244
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has suggested the crucial role of the dysregulation of microRNAs (miRNAs) in osteosarcoma (OS) progression. MicroRNA (miR)‑330‑5p has been reported to exert tumor suppressive effects in various types of human cancer. However, the role of miR‑330‑5p in the development of OS and the underlying mechanism remain to be clarified. In the present study, miR‑330‑5p expression was found to be significantly decreased in OS tissues and cell lines. In addition, low miR‑330‑5p expression was highly correlated with the overall survival and clinical stage of OS. Overexpression of miR‑330‑5p inhibited the viability, migration and invasion, and promoted the apoptosis of OS cells, as well as induced cell cycle arrest at the G2/M phase. Subsequently, the proto‑oncogene survivin was identified as a functional target of miR‑330‑5p, and this was validated using a luciferase reporter assay. It was also demonstrated that survivin expression was markedly increased in OS tissues, and that it was negatively correlated with the expression of miR‑330‑5p. Furthermore, overexpression of survivin significantly abrogated the tumor‑suppressive effect induced by miR‑330‑5p on OS cells. In conclusion, these results revealed that the miR‑330‑5p/survivin axis has a significant tumor‑suppressive effect on OS, and may serve as a diagnostic and therapeutic target for the treatment of OS.

References

1 

Kobayashi E, Hornicek FJ and Duan Z: MicroRNA involvement in osteosarcoma. Sarcoma. 2012:3597392012. View Article : Google Scholar : PubMed/NCBI

2 

Bruland OS, Bauer H, Alvegaard T and Smeland S: Treatment of osteosarcoma. The Scandinavian Sarcoma Group experience. Cancer Treat Res. 152:309–318. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Lagos-Quintana M, Rauhut R, Lendeckel W and Tuschl T: Identification of novel genes coding for small expressed RNAs. Science. 294:853–858. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Huang Y, Shen XJ, Zou Q, Wang SP, Tang SM and Zhang GZ: Biological functions of microRNAs: A review. J Physiol Biochem. 67:129–139. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Sotillo E and Thomas-Tikhonenko A: Shielding the messenger (RNA): microRNA-based anticancer therapies. Pharmacol Ther. 131:18–32. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Peng N, Miao Z, Wang L, Liu B, Wang G and Guo X: MiR-378 promotes the cell proliferation of osteosarcoma through down-regulating the expression of Kruppel-like factor 9. Biochem Cell Biol. 96:515–521. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Liu Z, Li Q, Zhao X, Cui B, Zhang L and Wang Q: MicroRNA-935 inhibits proliferation and invasion of osteosarcoma cells by directly targeting High mobility group box 1. Oncol Res. Feb 22–2018. View Article : Google Scholar

9 

Yang X, Wang L, Wang Q, Li L, Fu Y and Sun J: MiR-183 inhibits osteosarcoma cell growth and invasion by regulating LRP6-Wnt/β-catenin signaling pathway. Biochem Biophys Res Commun. 496:1197–1203. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Dong J, Liu Y, Liao W, Liu R, Shi P and Wang L: miRNA-223 is a potential diagnostic and prognostic marker for osteosarcoma. J Bone Oncol. 5:74–79. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Feng L, Ma J, Ji H, Liu Y and Hu W: miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5. Biosci Rep. 37(pii): BSR201700192017. View Article : Google Scholar : PubMed/NCBI

12 

Trehoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J, Jonckheere N and Van Seuningen I: Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta. 1853:2392–2403. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Wang H, Chen SH, Kong P, Zhang LY, Zhang LL, Zhang NQ and Gu H: Increased expression of miR-330-3p: A novel independent indicator of poor prognosis in human breast cancer. Eur Rev Med Pharmacol Sci. 22:1726–1730. 2018.PubMed/NCBI

14 

Su BB, Zhou SW, Gan CB and Zhang XN: MiR-330-5p regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma. J Surg Res. 203:434–440. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Wei CH, Wu G, Cai Q, Gao XC, Tong F, Zhou R, Zhang RG, Dong JH, Hu Y and Dong XR: MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway. J Hematol Oncol. 10:1252017. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Kim BK, Yoo HI, Choi K and Yoon SK: miR-330-5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression. FEBS J. 282:4692–4702. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Kong R, Liu W, Guo Y, Feng J, Cheng C, Zhang X, Ma Y, Li S, Jiang J, Zhang J, et al: Inhibition of NOB1 by microRNA-330-5p overexpression represses cell growth of non-small cell lung cancer. Oncol Rep. 38:2572–2580. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Lee KH, Chen YL, Yeh SD, Hsiao M, Lin JT, Goan YG and Lu PJ: MicroRNA-330 acts as tumor suppressor and induces apoptosis of prostate cancer cells through E2F1-mediated suppression of Akt phosphorylation. Oncogene. 28:3360–3370. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Qu S, Yao Y, Shang C, Xue Y, Ma J, Li Z and Liu Y: MicroRNA-330 is an oncogenic factor in glioblastoma cells by regulating SH3GL2 gene. PLoS One. 7:e460102012. View Article : Google Scholar : PubMed/NCBI

21 

Yao Y, Xue Y, Ma J, Shang C, Wang P, Liu L, Liu W, Li Z, Qu S, Li Z and Liu Y: MiR-330-mediated regulation of SH3GL2 expression enhances malignant behaviors of glioblastoma stem cells by activating ERK and PI3K/AKT signaling pathways. PLoS One. 9:e950602014. View Article : Google Scholar : PubMed/NCBI

22 

Luk SU, Xue H, Cheng H, Lin D, Gout PW, Fazli L, Collins CC, Gleave ME and Wang Y: The BIRC6 gene as a novel target for therapy of prostate cancer: Dual targeting of inhibitors of apoptosis. Oncotarget. 5:6896–6908. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Kusner LL, Ciesielski MJ, Marx A, Kaminski HJ and Fenstermaker RA: Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: A human and animal model study. PLoS One. 9:e1022312014. View Article : Google Scholar : PubMed/NCBI

24 

Wang HX, Chen G, Li GL and Jiang YJ: Expression and significance of Survivin and Smac in ovarian mucinous tumors. Zhonghua Bing Li Xue Za Zhi. 39:387–390. 2010.(In Chinese). PubMed/NCBI

25 

Wang H and Ye YF: Effect of survivin siRNA on biological behaviour of breast cancer MCF7 cells. Asian Pac J Trop Med. 8:225–228. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Chen X, Chen XG, Hu X, Song T, Ou X and Zhang C, Zhang W and Zhang C: MiR-34a and miR-203 Inhibit survivin expression to control cell proliferation and survival in human osteosarcoma cells. J Cancer. 7:1057–1065. 2016. View Article : Google Scholar : PubMed/NCBI

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September 2019
Volume 20 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
APA
Wang, H., Liu, L., & Fang, S. (2019). MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin. Molecular Medicine Reports, 20, 2236-2244. https://doi.org/10.3892/mmr.2019.10447
MLA
Wang, H., Liu, L., Fang, S."MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin". Molecular Medicine Reports 20.3 (2019): 2236-2244.
Chicago
Wang, H., Liu, L., Fang, S."MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin". Molecular Medicine Reports 20, no. 3 (2019): 2236-2244. https://doi.org/10.3892/mmr.2019.10447