Open Access

Sulforaphane prevents PC12 cells from oxidative damage via the Nrf2 pathway

  • Authors:
    • Bing Bao
    • Man‑Qing Zhang
    • Zhi‑Ying Chen
    • Xiang‑Bing Wu
    • Zhong‑Bing Xia
    • Jing‑Yan Chai
    • Xiao‑Ping Yin
  • View Affiliations

  • Published online on: April 10, 2019     https://doi.org/10.3892/mmr.2019.10148
  • Pages: 4890-4896
  • Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of this study was to investigate the protective effect of sulforaphane (SFN) on 1‑methyl‑4‑phenyl pyridine ion (MPP+)‑induced cytotoxicity and to investigate its possible mechanisms. Methods: PC12 cell toxicity induced by MPP+ served as a cell model of Parkinson's diseases. The cell culture + experiments were divided into four groups based on the different treatments, namely, vehicle control, SFN, MPP+ and SFN pretreatment plus MPP+. Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Expressions of nuclear factor erythroid 2‑related factor 2 (Nrf2), heme oxygenase 1 (HO‑1) and nicotinamide quinone oxidoreductase 1 (NQO1) were detected using western blotting. Results: MPP+ reduced the survival rate of PC12 cells in a dose‑ and time‑dependent manner. After 24‑h treatment with 500 µmol/l MPP+, the survival rate of PC12 cells decreased to 58.2±0.03% of that in the control groups. Under the same conditions MPP+ resulted in significant apoptosis of PC12 cells (apoptosis rate: 30.4±0.6%). However, SFN pretreatment significantly attenuated the cell damage induced by MPP+. Furthermore, it was demonstrated that SFN reversed the reduction of Nrf2, HO‑1 and NQO1 expression induced by MPP+. Conclusion: SFN may protect PC12 cells from MPP+‑induced damage via activating the Nrf2‑ARE (antioxidant responsive element) pathway.
View Figures
View References

Related Articles

Journal Cover

June 2019
Volume 19 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Bao, B., Zhang, M., Chen, Z., Wu, X., Xia, Z., Chai, J., & Yin, X. (2019). Sulforaphane prevents PC12 cells from oxidative damage via the Nrf2 pathway. Molecular Medicine Reports, 19, 4890-4896. https://doi.org/10.3892/mmr.2019.10148
MLA
Bao, B., Zhang, M., Chen, Z., Wu, X., Xia, Z., Chai, J., Yin, X."Sulforaphane prevents PC12 cells from oxidative damage via the Nrf2 pathway". Molecular Medicine Reports 19.6 (2019): 4890-4896.
Chicago
Bao, B., Zhang, M., Chen, Z., Wu, X., Xia, Z., Chai, J., Yin, X."Sulforaphane prevents PC12 cells from oxidative damage via the Nrf2 pathway". Molecular Medicine Reports 19, no. 6 (2019): 4890-4896. https://doi.org/10.3892/mmr.2019.10148