Open Access

Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture

  • Authors:
    • Zhenglu Wang
    • Bowen Bi
    • Hongli Song
    • Lei Liu
    • Hong Zheng
    • Shusen Wang
    • Zhongyang Shen
  • View Affiliations

  • Published online on: April 12, 2019     https://doi.org/10.3892/mmr.2019.10160
  • Pages: 4623-4630
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer mortality worldwide, which is partially due to the lack of appropriate therapeutic options. The development of HCC is accompanied with unique and continuous genomic and epigenetic modifications. Therefore, the absence of a personalized and reproducible human model reduces the ability to determine the potential of candidate treatments. Conditional reprogramming (CR) culture has been used to establish and indefinitely grow patient‑derived tumor cell lines in a rapid and efficient manner. In the present study, primary HCC cells were isolated from tumor specimens and cultured under CR conditions. The proliferative potential and capacity of cells to undergo continuous regeneration were evaluated by cell viability and proliferation assays, and the expression of tumor‑specific markers was determined by western blotting and immunofluorescence to determine the prospects for use in clinical settings. It was demonstrated that ~55% of tumor samples were able to generate HCC cells that could be continuously expanded and passaged under CR conditions; this ability was associated with the source and composition of the tumor tissues. Furthermore, the expression of the tumor‑specific marker α‑fetoprotein and the proliferative ability of cells were maintained following cycles of cryopreservation and resuscitation. In conclusion, with further optimization, the CR system may be a useful tool for the precise therapeutic treatment of patients with HCC.

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June 2019
Volume 19 Issue 6

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Copy and paste a formatted citation
APA
Wang, Z., Bi, B., Song, H., Liu, L., Zheng, H., Wang, S., & Shen, Z. (2019). Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture. Molecular Medicine Reports, 19, 4623-4630. https://doi.org/10.3892/mmr.2019.10160
MLA
Wang, Z., Bi, B., Song, H., Liu, L., Zheng, H., Wang, S., Shen, Z."Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture". Molecular Medicine Reports 19.6 (2019): 4623-4630.
Chicago
Wang, Z., Bi, B., Song, H., Liu, L., Zheng, H., Wang, S., Shen, Z."Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture". Molecular Medicine Reports 19, no. 6 (2019): 4623-4630. https://doi.org/10.3892/mmr.2019.10160