Hydroxytyrosol promotes autophagy by regulating SIRT1 against advanced oxidation protein product‑induced NADPH oxidase and inflammatory response

  • Authors:
    • Tian Sun
    • Qian Chen
    • Si‑Yuan Zhu
    • Qian Wu
    • Cong‑Rui Liao
    • Zheng Wang
    • Xiao‑Hu Wu
    • Hang‑Tian Wu
    • Jian‑Ting Chen
  • View Affiliations

  • Published online on: August 5, 2019     https://doi.org/10.3892/ijmm.2019.4300
  • Pages: 1531-1540
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Abstract

Advanced oxidation protein products (AOPPs) can trigger NADPH oxidase (NOX) and lead to the production of reactive oxygen species (ROS) in the pathophysiology of rheumatoid arthritis (RA). Hydroxytyrosol (HT) is a phenolic composite in olive oil that has antioxidant and anti‑inflammatory effects and enhances autophagy. Early research has revealed that HT can activate the silent information regulator 1 (SIRT1) pathway to induce autophagy and alleviate the cartilage inflammatory response caused by H2O2. However, whether HT can attenuate AOPP‑induced NOX and inflammatory responses remains to be elucidated. The present study aimed to investigate how HT can alleviate the damage caused by AOPPs. In cell experiments, chondrocytes were pre‑stimulated with HT and then exposed to AOPPs. First, it was found that HT promoted autophagy through the SIRT1 pathway, increased the expression of autophagy‑related proteins including microtubule‑associated protein 1 light chain 3, autophagy related (ATG)5 and ATG7, and decreased the expression of P62. Furthermore, HT reduced the expression of NOX, which was affected by AOPPs in chondrocytes through the SIRT1 pathway. Finally, the expression of inflammatory cytokines caused by AOPPs was downregulated following HT treatment. In conclusion, it was found that HT reduced the expression of NOX and inhibited the inflammatory response caused by AOPPs in chondrocytes through the SIRT1 pathway.

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October 2019
Volume 44 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Sun, T., Chen, Q., Zhu, S., Wu, Q., Liao, C., Wang, Z. ... Chen, J. (2019). Hydroxytyrosol promotes autophagy by regulating SIRT1 against advanced oxidation protein product‑induced NADPH oxidase and inflammatory response. International Journal of Molecular Medicine, 44, 1531-1540. https://doi.org/10.3892/ijmm.2019.4300
MLA
Sun, T., Chen, Q., Zhu, S., Wu, Q., Liao, C., Wang, Z., Wu, X., Wu, H., Chen, J."Hydroxytyrosol promotes autophagy by regulating SIRT1 against advanced oxidation protein product‑induced NADPH oxidase and inflammatory response". International Journal of Molecular Medicine 44.4 (2019): 1531-1540.
Chicago
Sun, T., Chen, Q., Zhu, S., Wu, Q., Liao, C., Wang, Z., Wu, X., Wu, H., Chen, J."Hydroxytyrosol promotes autophagy by regulating SIRT1 against advanced oxidation protein product‑induced NADPH oxidase and inflammatory response". International Journal of Molecular Medicine 44, no. 4 (2019): 1531-1540. https://doi.org/10.3892/ijmm.2019.4300