Open Access

Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus

  • Authors:
    • Qing Luo
    • Lu Zhang
    • Xue Li
    • Biqi Fu
    • Yang Guo
    • Zikun Huang
    • Junming Li
  • View Affiliations

  • Published online on: August 5, 2019     https://doi.org/10.3892/ijmm.2019.4302
  • Pages: 1462-1472
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) have emerged as novel biomarkers for disease diagnosis. However, the expression profiles and clinical significance of circRNAs in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) remain unclear. In the present study, the expression profile of circRNAs in PBMCs from patients with SLE and healthy controls (HCs) was detected by using microarray analysis and verified by reverse transcription‑quantitative polymerase chain reaction. A total of 1,603 circRNAs were identified to be significantly aberrantly expressed in PBMCs from patients with SLE. Validation assays in 30 SLE patients and 20 HCs demonstrated that the levels of hsa_circ_0044235 and hsa_circ_0068367 were significantly decreased in the patients with SLE. Receiver operating characteristic curve analysis suggested that hsa_circ_0044235 and hsa_circ_0068367 were significant for SLE diagnosis. Furthermore, the diagnostic potential of hsa_circ_0044235 and hsa_circ_0068367 for SLE was validated in an independent validation set with 45 patients with SLE, 38 HCs and 30 patients with rheumatoid arthritis. In addition, the level of hsa_circ_0044235 in the PBMCs from patients with SLE were identified to be significantly increased in new‑onset SLE patients and in patients who were determined to be positive for anti‑double‑stranded DNA and anti‑ribosomal protein P antibodies. Additionally, the level of a microRNA (miRNA) target of hsa_circ_0044235, hsa‑miRNA‑892a, was identified to be significantly increased in the PBMCs from patients with SLE. The present study suggested that the dysregulation of circRNAs may serve a role in SLE pathogenesis, and that the levels of hsa_circ_0044235 and hsa_circ_0068367 in PBMC have potential as biomarkers for SLE diagnosis.

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October 2019
Volume 44 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Luo, Q., Zhang, L., Li, X., Fu, B., Guo, Y., Huang, Z., & Li, J. (2019). Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus. International Journal of Molecular Medicine, 44, 1462-1472. https://doi.org/10.3892/ijmm.2019.4302
MLA
Luo, Q., Zhang, L., Li, X., Fu, B., Guo, Y., Huang, Z., Li, J."Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus". International Journal of Molecular Medicine 44.4 (2019): 1462-1472.
Chicago
Luo, Q., Zhang, L., Li, X., Fu, B., Guo, Y., Huang, Z., Li, J."Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus". International Journal of Molecular Medicine 44, no. 4 (2019): 1462-1472. https://doi.org/10.3892/ijmm.2019.4302