Open Access

Dissociation of HKII in retinal epithelial cells induces oxidative stress injury in the retina

  • Authors:
    • Liqun Chu
    • Lin Xiao
    • Bing Xu
    • Jingmei Xu
  • View Affiliations

  • Published online on: August 6, 2019     https://doi.org/10.3892/ijmm.2019.4304
  • Pages: 1377-1387
  • Copyright: © Chu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The retina is sensitive to injury resulting from oxidative stress (OS) due to its high oxygen consumption. Patients with retinitis pigmentosa suffer from excessive OS. N‑acetylcysteine (NAC) is used as a mucolytic agent for the clinical treatment of disorders, such as chronic bronchitis and other pulmonary diseases. The aim of the present study was to investigate the role of hexokinase 2 (HKII) in retinal OS injury. Amyloid β (Aβ)1‑40 was used to establish a cellular model of OS. Cell viability was measured with a Cell Counting Kit‑8 assay, and the apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) of cells were analyzed via flow cytometry with corresponding kits. The mRNA and protein levels were detected by reverse transcription‑quantitative PCR and western blot analyses, respectively. It was observed that Aβ1‑40 reduced the expression of HKII in the mitochondria of retinal pigment epithelial ARPE cells and impaired mitochondrial antioxidant functions. Additionally, knockdown of HKII promoted apoptosis, and increased ROS levels and the MMP. NAC attenuated the inhibition of mitochondrial functions induced by Aβ1‑40. The knockdown of HKII was revealed to decrease the levels of Bcl‑2, manganese superoxide dismutase (SOD) and copper‑zinc‑SOD, and increase the levels of cleaved caspase‑3, Bax and cytochrome c. The present findings suggested that the dissociation of HKII induced by OS induces apoptosis and mitochondrial damage. This study provided improved understanding of the mechanisms underlying the effects of OS on retinal epithelial cells.

Related Articles

Journal Cover

October 2019
Volume 44 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Chu, L., Xiao, L., Xu, B., & Xu, J. (2019). Dissociation of HKII in retinal epithelial cells induces oxidative stress injury in the retina. International Journal of Molecular Medicine, 44, 1377-1387. https://doi.org/10.3892/ijmm.2019.4304
MLA
Chu, L., Xiao, L., Xu, B., Xu, J."Dissociation of HKII in retinal epithelial cells induces oxidative stress injury in the retina". International Journal of Molecular Medicine 44.4 (2019): 1377-1387.
Chicago
Chu, L., Xiao, L., Xu, B., Xu, J."Dissociation of HKII in retinal epithelial cells induces oxidative stress injury in the retina". International Journal of Molecular Medicine 44, no. 4 (2019): 1377-1387. https://doi.org/10.3892/ijmm.2019.4304