Open Access

Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis

  • Authors:
    • Hui Jiang
    • Fu‑Rong Wu
    • Jian Liu
    • Xiu‑Juan Qin
    • Nan‑Nan Jiang
    • Wei‑Ping Li
  • View Affiliations

  • Published online on: July 22, 2019     https://doi.org/10.3892/ijmm.2019.4281
  • Pages: 1344-1356
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology, which occurs in ~1.0% of the general population. Increasing studies have suggested that long non‑coding RNAs (lncRNAs) may serve important roles in various biological processes and may be associated with the pathogenesis of different types of disease, including RA. Astragalosides (AST) has been used as a traditional Chinese medicine for the treatment of RA. However, the mechanism underlying its therapeutic effect has remained unclear to date. Thus, there is an urgent need to elucidate the possible mechanism of AST in the treatment of RA from the perspective of lncRNAs. In the present study, the lncRNAs and mRNAs of a vehicle group, animal model group and AST treatment (control) group were determined by Arraystar Rat lncRNA/mRNA microarray. The differentially expressed genes with a fold change >1.5 and P<0.05 were selected and analyzed. Gene Ontology (GO) and pathway analysis was performed using the Database for Annotation, Visualization and Integration Discovery, and the coding‑non‑coding gene co‑expression network was drawn based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. Based on node degree and the correlation between bioinformatics analysis and RA, the critical differentially expressed lncRNAs were selected, analyzed and verified by reverse transcription‑quantitative PCR (RT‑qPCR) analysis. The results showed that, following AST treatment, up to 75 lncRNAs and 247 mRNAs were found to be differentially expressed among the three groups. GO and pathway analysis manifested that 135 GO terms and 17 pathways were enriched by differentially expressed genes. Four lncRNAs (MRAK012530, MRAK132628, MRAK003448 and XR_006457) were selected as the critical lncRNAs and their trend in expression showed consistency between the RT‑qPCR and microarray data. In conclusion, AST had a regulatory effect on differentially expressed lncRNAs during the development of RA, and four lncRNAs could be selected as critical therapeutic targets of AST.

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October 2019
Volume 44 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Jiang, H., Wu, F., Liu, J., Qin, X., Jiang, N., & Li, W. (2019). Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis. International Journal of Molecular Medicine, 44, 1344-1356. https://doi.org/10.3892/ijmm.2019.4281
MLA
Jiang, H., Wu, F., Liu, J., Qin, X., Jiang, N., Li, W."Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis". International Journal of Molecular Medicine 44.4 (2019): 1344-1356.
Chicago
Jiang, H., Wu, F., Liu, J., Qin, X., Jiang, N., Li, W."Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis". International Journal of Molecular Medicine 44, no. 4 (2019): 1344-1356. https://doi.org/10.3892/ijmm.2019.4281