Open Access

Expression changes of CX3CL1 and CX3CR1 proteins in the hippocampal CA1 field of the gerbil following transient global cerebral ischemia

  • Authors:
    • Ji Hyeon Ahn
    • Dae Won Kim
    • Joon Ha Park
    • Tae‑Kyeong Lee
    • Hyang‑Ah Lee
    • Moo‑Ho Won
    • Choong‑Hyun Lee
  • View Affiliations

  • Published online on: July 10, 2019     https://doi.org/10.3892/ijmm.2019.4273
  • Pages: 939-948
  • Copyright: © Ahn et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chemokine C‑X3‑C motif ligand 1 (CX3CL1) and its sole receptor, CX3CR1, are known to be involved in neuronal damage/death following brain ischemia. In the present study, time‑dependent expression changes of CX3CL1 and CX3CR1 proteins were investigated in the hippocampal CA1 field following 5 min of transient global cerebral ischemia (tgCI) in gerbils. To induce tgCI in gerbils, bilateral common carotid arteries were occluded for 5 min using aneurysm clips. Expression changes of CX3CL1 and CX3CR1 proteins were assessed at 1, 2 and 5 days after tgCI using western blotting and immunohistochemistry. CX3CL1 immunoreactivity was strong in the CA1 pyramidal cells of animals in the sham operation group. Weak CX3CL1 immunoreactivity was detected at 6 h after tgCI, recovered at 1 day after tgCI and disappeared from 5 days after tgCI. CX3CR1 immunoreactivity was very weak in CA1 pyramidal cells of the sham animals. CX3CR1 immunoreactivity in CA1 pyramidal cells was significantly increased at 1 days after tgCI and gradually decreased thereafter. On the other hand, CX3CR1 immunoreactivity was significantly increased in microglia from 5 days after tgCI. These results showed that CX3CL1 and CX3CR1 protein expression levels in pyramidal cells and microglia in the hippocampal CA1 field following tgCI were changed, indicating that tgCI‑induced expression changes of CX3CL1 and CX3CR1 proteins might be closely associated with tgCI‑induced delayed neuronal death and microglial activation.

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Copy and paste a formatted citation
APA
Ahn, J.H., Kim, D.W., Park, J.H., Lee, T., Lee, H., Won, M., & Lee, C. (2019). Expression changes of CX3CL1 and CX3CR1 proteins in the hippocampal CA1 field of the gerbil following transient global cerebral ischemia. International Journal of Molecular Medicine, 44, 939-948. https://doi.org/10.3892/ijmm.2019.4273
MLA
Ahn, J. H., Kim, D. W., Park, J. H., Lee, T., Lee, H., Won, M., Lee, C."Expression changes of CX3CL1 and CX3CR1 proteins in the hippocampal CA1 field of the gerbil following transient global cerebral ischemia". International Journal of Molecular Medicine 44.3 (2019): 939-948.
Chicago
Ahn, J. H., Kim, D. W., Park, J. H., Lee, T., Lee, H., Won, M., Lee, C."Expression changes of CX3CL1 and CX3CR1 proteins in the hippocampal CA1 field of the gerbil following transient global cerebral ischemia". International Journal of Molecular Medicine 44, no. 3 (2019): 939-948. https://doi.org/10.3892/ijmm.2019.4273