Open Access

Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis

  • Authors:
    • Ye Yang
    • Xinjing Du
    • Qiang Wang
    • Jianwei Liu
    • Enguo Zhang
    • Linlin Sai
    • Cheng Peng
    • Martin F. Lavin
    • Abrey Jie Yeo
    • Xu Yang
    • Hua Shao
    • Zhongjun Du
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/ijmm.2019.4265
  • Pages: 903-912
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Silicon is one of the most widely used chemical materials, and the increasing use of silica nanoparticles (SNs) highlights the requirement for safety and biological toxicity studies. The damaging and adverse effects of SNs on human hepatocytes remain largely unknown, as do the mechanisms involved. In the present study, the mechanisms underlying SN‑induced toxicity in the human hepatocyte cell line HL‑7702 were investigated. An MTT assay revealed that following exposure to SNs in the concentration range of 25‑200 µg/ml, the viability of HL‑7702 cells decreased, and the viability decreased further with increasing exposure time. SNs induced a delay in the S and G2/M phases of the cell cycle, and also induced DNA damage in these cells. Western blot and flow cytometry analyses revealed that cell death was mediated by mitochondrial damage and the upregulated expression of a number of pro‑apoptotic proteins. In conclusion, exposure to SNs led to mitochondrial and DNA damage, resulting in apoptosis‑mediated HL‑7702 cell death. The study provided evidence for the cellular toxicity of SNs, and added to the growing body of evidence regarding the potential damaging effects of nanoparticles, indicating that caution should be exercised in their widespread usage.
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September 2019
Volume 44 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Yang, Y., Du, X., Wang, Q., Liu, J., Zhang, E., Sai, L. ... Du, Z. (2019). Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis. International Journal of Molecular Medicine, 44, 903-912. https://doi.org/10.3892/ijmm.2019.4265
MLA
Yang, Y., Du, X., Wang, Q., Liu, J., Zhang, E., Sai, L., Peng, C., Lavin, M. F., Yeo, A. J., Yang, X., Shao, H., Du, Z."Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis". International Journal of Molecular Medicine 44.3 (2019): 903-912.
Chicago
Yang, Y., Du, X., Wang, Q., Liu, J., Zhang, E., Sai, L., Peng, C., Lavin, M. F., Yeo, A. J., Yang, X., Shao, H., Du, Z."Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis". International Journal of Molecular Medicine 44, no. 3 (2019): 903-912. https://doi.org/10.3892/ijmm.2019.4265