Open Access

Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis

  • Authors:
    • Tao Wang
    • Yize Liu
    • Yong Wang
    • Xuyang Huang
    • Wei Zhao
    • Zhonghai Zhao
  • View Affiliations

  • Published online on: June 12, 2019     https://doi.org/10.3892/ijmm.2019.4240
  • Pages: 630-642
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is a common and troublesome disease among the elderly, and is characterized by extracellular matrix (ECM) degradation. The function of the long non‑coding RNA X‑inactive‑specific transcript (XIST) and its working mechanism in ECM degradation remains unclear. In the present study, XIST was revealed to be upregulated in OA specimens and in articular chondrocytes (ACs) derived from OA tissue (AC/OA) and interleukin‑1β (IL‑1β)‑treated ACs. Loss‑of‑function experiments demonstrated that downregulation of XIST suppressed the degradation of the ECM in AC/OA and AC/IL‑1β‑5.0 cells. Furthermore, XIST, matrix metalloproteinase 13 (MMP‑13) and ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS5) were identified as targets of microRNA (miR)‑1277‑5p, and the reciprocal inhibitive effect between XIST and miR‑1277‑5p was elucidated. Furthermore, the role of XIST in ECM degradation was confirmed to be functioning as a competing endogenous RNA (ceRNA) of miR‑1277‑5p. Finally, the protective effect of the downregulation of XIST on ECM degradation was verified in an OA rat model. In conclusion, the present study suggests that XIST promotes MMP‑13 and ADAMTS5 expression, indicating ECM degradation, by functioning as a ceRNA of miR‑1277‑5p in OA. The present study proposed a novel potential target with a new working mechanism in molecular treating of OA.

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August 2019
Volume 44 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
APA
Wang, T., Liu, Y., Wang, Y., Huang, X., Zhao, W., & Zhao, Z. (2019). Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis. International Journal of Molecular Medicine, 44, 630-642. https://doi.org/10.3892/ijmm.2019.4240
MLA
Wang, T., Liu, Y., Wang, Y., Huang, X., Zhao, W., Zhao, Z."Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis". International Journal of Molecular Medicine 44.2 (2019): 630-642.
Chicago
Wang, T., Liu, Y., Wang, Y., Huang, X., Zhao, W., Zhao, Z."Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis". International Journal of Molecular Medicine 44, no. 2 (2019): 630-642. https://doi.org/10.3892/ijmm.2019.4240