Open Access

Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion

  • Authors:
    • Qian Wang
    • Zhi‑Xiao Li
    • Yu‑Juan Li
    • Zhi‑Gang He
    • Ying‑Le Chen
    • Mao‑Hui Feng
    • Shun‑Yuan Li
    • Duo‑Zhi Wu
    • Hong‑Bing Xiang
  • View Affiliations

  • Published online on: March 29, 2019     https://doi.org/10.3892/ijmm.2019.4151
  • Pages: 2361-2375
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The identification of the expression patterns of long non‑coding RNAs (lncRNAs) and mRNAs in the spinal cord under normal and cardiac ischemia/reperfusion (I/R) conditions is essential for understanding the genetic mechanisms underlying the pathogenesis of cardiac I/R injury. The present study used high‑throughput RNA sequencing to investigate differential gene and lncRNA expression patterns in the spinal cords of rats during I/R‑induced cardiac injury. Male Sprague Dawley rats were assigned to the following groups: i) Control; ii) 2 h (2 h post‑reperfusion); and iii)v0.5 h (0.5 h post‑reperfusion). Further mRNA/lncRNA microarray analysis revealed that the expression profiles of lncRNA and mRNA in the spinal cords differed markedly between the control and 2 h groups, and in total 7,980 differentially expressed (>2‑fold) lncRNAs (234 upregulated, 7,746 downregulated) and 3,428 mRNAs (767 upregulated, 2,661 downregulated) were identified. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to determine the expression patterns of several lncRNAs. The results indicated that the expression levels of lncRNA NONRATT025386 were significantly upregulated in the 2 and 0.5 h groups when compared with those in the control group, whereas the expression levels of NONRATT016113, NONRATT018298 and NONRATT018300 were elevated in the 2 h group compared with those in the control group; however, there was no statistically significant difference between the 0.5 h and control groups. Furthermore, the expression of lncRNA NONRATT002188 was significantly downregulated in the 0.5 and 2 h groups when compared with the control group. The present study determined the expression pattern of lncRNAs and mRNAs in rat spinal cords during cardiac I/R. It was suggested that lncRNAs and mRNAs from spinal cords may be novel therapeutic targets for the treatment of I/R‑induced cardiac injury.
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June 2019
Volume 43 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Wang, Q., Li, Z., Li, Y., He, Z., Chen, Y., Feng, M. ... Xiang, H. (2019). Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion . International Journal of Molecular Medicine, 43, 2361-2375. https://doi.org/10.3892/ijmm.2019.4151
MLA
Wang, Q., Li, Z., Li, Y., He, Z., Chen, Y., Feng, M., Li, S., Wu, D., Xiang, H."Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion ". International Journal of Molecular Medicine 43.6 (2019): 2361-2375.
Chicago
Wang, Q., Li, Z., Li, Y., He, Z., Chen, Y., Feng, M., Li, S., Wu, D., Xiang, H."Identification of lncRNA and mRNA expression profiles in rat spinal cords at various time‑points following cardiac ischemia/reperfusion ". International Journal of Molecular Medicine 43, no. 6 (2019): 2361-2375. https://doi.org/10.3892/ijmm.2019.4151