Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection
- Authors:
- Keiko Ikeda
- Hisashi Yamasaki
- Sawako Minami
- Yukiko Suzuki
- Kazuko Tsujimoto
- Yoshihisa Sekino
- Hiroshi Irie
- Tsutomu Arakawa
- A. Hajime Koyama
View Affiliations
Affiliations: Department of Cellular and Molecular Medicine, Division of Virology, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, Department of Reproductive and Developmental Medicine, Division of Reproductive Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, School of Health and Nursing Science, Wakayama Medical University, Wakayama 641-8509, Japan, Wakayama Shin-ai Women's Junior College, Wakayama 640-0341, Japan, Department of Anatomy, School of Medicine, Teikyo University, Itabashi 173-8605, Japan, Department of Sport and Medical Science, School of Medical Technology, Teikyo University, Itabashi 173-8605, Japan, Alliance Protein Laboratories, Thousand Oaks, CA 91360, USA
-
Published online on: October 4, 2012
https://doi.org/10.3892/ijmm.2012.1149
-
Pages: 1307-1312
Metrics:
Total Views:
0
(Spandidos Publications:
| PMC Statistics:
)
Metrics:
Total PDF Downloads:
0
(Spandidos Publications:
| PMC Statistics:
)
This article is mentioned in:
Abstract
Arginine, among the amino acids, has demonstrated unique properties, including suppression of protein-protein interactions and virus inactivation. We investigated the effects of arginine on the infectivity of human herpesvirus 2 (HHV-2) and the potential application of arginine as a chemotherapeutic agent against genital herpes. Arginine directly inactivated HHV-2 and characterization of the inactivation demonstrated that 1 M arginine at pH 4.3 inactivated the virus more efficiently compared to 0.1 M citrate or 1 M sodium chloride, indicating that neither acidic pH nor ionic strength alone is sufficient for virus inactivation. The effect of arginine was rapid and concentration-dependent. Although virus inactivation was efficient at an acidic pH, arginine inactivated the virus even at a neutral pH, provided that a higher arginine concentration and prolonged incubation time were used. In addition, arginine suppressed the multiplication of HHV-2 under the conditions at which its effect on cell viability was insignificant. Pilot mouse model studies revealed a marked suppression of death by arginine when the mice were infected with HHV-2 through the vaginal route, followed by an intermittent application of acidic arginine by vaginal instillation.
View Figures |
Figure 1
|
 |
Figure 2
|
 |
Figure 3
|
 |
Figure 4
|
 |
Figure 5
|
 |
Figure 6
|
 |
Figure 7
|
View References
|
1.
|
T ArakawaM UozakiAH KoyamaModulation of
small molecule solubility and protein binding by arginineMol Med
Rep3833836201021472322
|
|
2.
|
H YamasakiK TsujimotoAH KoyamaD EjimaT
ArakawaArginine facilitates inactivation of enveloped virusesJ
Pharm Sci9730673073200810.1002/jps.2122418186461
|
|
3.
|
H UtsunomiyaM IchinoseK TsujimotoY
KatsuyamaH YamasakiAH KoyamaD EjimaT ArakawaCo-operative thermal
inactivation of human herpes simplex virus and influenza virus by
arginine and NaClInt J
Pharm36699102200910.1016/j.ijpharm.2008.09.01218845231
|
|
4.
|
T ArakawaY KitaAH KoyamaSynergistic virus
inactivation effects of arginineBiotechnol
J4174178200910.1002/biot.20080017719156749
|
|
5.
|
K TsujimotoM UozakiK IkedaH YamasakiH
UtsunomiyaM IchinoseAH KoyamaT ArakawaSolvent-induced virus
inactivation by acidic arginine solutionInt J Mol
Med25433437201020127049
|
|
6.
|
Y KatsuyamaH YamasakiK TsujimotoAH KoyamaD
EjimaT ArakawaButyroyl-arginine as a potent virus inactivation
agentInt J
Pharm3619298200810.1016/j.ijpharm.2008.05.02018617337
|
|
7.
|
K BrorsonS KrejciK LeeE HamiltonK SteinY
XuBracketed generic inactivation of rodent retroviruses by low pH
treatment for monoclonal antibodies and recombinant
proteinsBiotechnol Bioeng82321329200310.1002/bit.1057412599259
|
|
8.
|
K IkedaH YamasakiY SuzukiAH KoyamaT
ArakawaNew strategy with acidic arginine solution for the treatment
of influenza A virus infection: ReviewExp Ther
Med1251256201022993536
|
|
9.
|
K IkedaH YamasakiS MinamiT NaitoH IrieT
ArakawaAH KoyamaVirucidal ability of arginine and its possible
application as an antiherpetic agent. In: From the Hallowed Halls
of HerpesvirologyJ BainesJ BlahoImperial College
PressLondon4354492011
|
|
10.
|
T NaitoH IrieK TsujimotoK IkedaT ArakawaAH
KoyamaAntiviral effect of arginine against herpes simplex virus
type 1Int J Mol Med23495499200910.3892/ijmm_0000015619288025
|
|
11.
|
B RoizmanDM KnipeRJ WhitleyHerpes simplex
virusesDM KnipePM HowleyFields Virology5th editionLippincott
Williams and WilkinsPhiladelphia250126012007
|
|
12.
|
AH KoyamaY MiwaSuppression of apoptotic
DNA fragmentation in herpes simplex virus type 1-infected cellsJ
Virol712567257119979032402
|
|
13.
|
AH KoyamaH AkariA AdachiF GoshimaY
NishiyamaInduction of apoptosis in HEp-2 cells by infection with
herpes simplex virus type 2Arch
Virol14324352441199810.1007/s0070500504739930199
|
|
14.
|
AH KoyamaT UchidaThe effect of ammonium
chloride on the multiplication of herpes simplex virus type 1 in
Vero cellsVirus
Res13271281198910.1016/0168-1702(89)90073-72554609
|
|
15.
|
AH KoyamaT ArakawaA AdachiAcceleration of
virus-induced apoptosis by tumor necrosis factorFEBS
Lett426179182199810.1016/S0014-5793(98)00338-X9599003
|
|
16.
|
S HataAH KoyamaH ShiotaA AdachiF GoshimaY
NishiyamaAntiapoptotic activity of herpes simplex virus type 2: A
role of US3 protein kinase geneMicrobe
Infect1601607199910.1016/S1286-4579(99)80059-810611736
|
|
17.
|
AH KoyamaT UchidaQuantitative studies on
the maturation process of herpes simplex virus type 1 in Vero
cellsVirus Res10281285198810.1016/0168-1702(88)90023-82842975
|
|
18.
|
D AroraN KhannaMethod for increasing the
yield of properly folded recombinant human gamma interferon from
inclusion bodiesJ
Biotechnol52127133199610.1016/S0168-1656(96)01636-79084211
|
|
19.
|
H TakadaC KishimotoY HiraokaH OchiaiOral
L-arginine prevents murine coxsackievirus B3 myocarditisInt J
Cardiol86272279200210.1016/S0167-5273(02)00279-612419566
|
|
20.
|
H AgawaK IkutaY MinamiyamaM InoueT
SairenjiDown-regulation of spontaneous Epstein-Barr virus
reactivation in the P3HR-1 cell line by
L-arginineVirology304114124200210.1006/viro.2002.170912490409
|
