Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

  • Authors:
    • Keiko Ikeda
    • Hisashi Yamasaki
    • Sawako Minami
    • Yukiko Suzuki
    • Kazuko Tsujimoto
    • Yoshihisa Sekino
    • Hiroshi Irie
    • Tsutomu Arakawa
    • A. Hajime Koyama
  • View Affiliations

  • Published online on: October 4, 2012     https://doi.org/10.3892/ijmm.2012.1149
  • Pages: 1307-1312
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Abstract

Arginine, among the amino acids, has demonstrated unique properties, including suppression of protein-protein interactions and virus inactivation. We investigated the effects of arginine on the infectivity of human herpesvirus 2 (HHV-2) and the potential application of arginine as a chemotherapeutic agent against genital herpes. Arginine directly inactivated HHV-2 and characterization of the inactivation demonstrated that 1 M arginine at pH 4.3 inactivated the virus more efficiently compared to 0.1 M citrate or 1 M sodium chloride, indicating that neither acidic pH nor ionic strength alone is sufficient for virus inactivation. The effect of arginine was rapid and concentration-dependent. Although virus inactivation was efficient at an acidic pH, arginine inactivated the virus even at a neutral pH, provided that a higher arginine concentration and prolonged incubation time were used. In addition, arginine suppressed the multiplication of HHV-2 under the conditions at which its effect on cell viability was insignificant. Pilot mouse model studies revealed a marked suppression of death by arginine when the mice were infected with HHV-2 through the vaginal route, followed by an intermittent application of acidic arginine by vaginal instillation.

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December 2012
Volume 30 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Ikeda, K., Yamasaki, H., Minami, S., Suzuki, Y., Tsujimoto, K., Sekino, Y. ... Koyama, A.H. (2012). Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection. International Journal of Molecular Medicine, 30, 1307-1312. https://doi.org/10.3892/ijmm.2012.1149
MLA
Ikeda, K., Yamasaki, H., Minami, S., Suzuki, Y., Tsujimoto, K., Sekino, Y., Irie, H., Arakawa, T., Koyama, A. H."Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection". International Journal of Molecular Medicine 30.6 (2012): 1307-1312.
Chicago
Ikeda, K., Yamasaki, H., Minami, S., Suzuki, Y., Tsujimoto, K., Sekino, Y., Irie, H., Arakawa, T., Koyama, A. H."Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection". International Journal of Molecular Medicine 30, no. 6 (2012): 1307-1312. https://doi.org/10.3892/ijmm.2012.1149