Open Access

MicroRNA‑146a inhibits NF‑κB activation and pro‑inflammatory cytokine production by regulating IRAK1 expression in THP‑1 cells

  • Authors:
    • Chunlei Zhou
    • Lan Zhao
    • Kai Wang
    • Qianru Qi
    • Meng Wang
    • Lei Yang
    • Ping Sun
    • Hong Mu
  • View Affiliations

  • Published online on: August 13, 2019     https://doi.org/10.3892/etm.2019.7881
  • Pages: 3078-3084
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miR)‑146a levels are reduced in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE); however, its function is not well understood. The present study investigated the role of miR‑146a in the regulation of lipopolysaccharide (LPS)‑induced inflammation in THP‑1 cells. A miR‑146a mimic and an inhibitor were used to overexpress and downregulate miR‑146a expression, respectively. Reverse transcription‑quantitative PCR and western blot analyses were performed to evaluate interleukin (IL)‑1 receptor‑associated kinase 1 (IRAK1) expression, and western blot analysis was applied to assess nuclear factor‑κB activation by analyzing p65 subunit levels in the nucleus. To investigate the effects of miR‑146a on LPS‑induced inflammation, IL‑6 and tumor necrosis factor‑α (TNF‑α) levels were also measured using ELISA. The results of the present study revealed thatmiR‑146a overexpression significantly reduced IRAK1 expression, reduced p65 levels in the nucleus and reduced IL‑6 and TNF‑α levels in the supernatant of the cell culture medium of THP‑1 cells following LPS treatment. Luciferase assays confirmed IRAK1 to be a direct target of miR‑146a in THP‑1 cells. In conclusion, miR‑146a may regulate IRAK1 expression and inhibit the activation of inflammatory signals and secretion of pro‑inflammatory cytokines. The present study revealed, at least in part, the mechanisms by which miR‑146a regulate the inflammatory response in SLE.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Zhou, C., Zhao, L., Wang, K., Qi, Q., Wang, M., Yang, L. ... Mu, H. (2019). MicroRNA‑146a inhibits NF‑κB activation and pro‑inflammatory cytokine production by regulating IRAK1 expression in THP‑1 cells. Experimental and Therapeutic Medicine, 18, 3078-3084. https://doi.org/10.3892/etm.2019.7881
MLA
Zhou, C., Zhao, L., Wang, K., Qi, Q., Wang, M., Yang, L., Sun, P., Mu, H."MicroRNA‑146a inhibits NF‑κB activation and pro‑inflammatory cytokine production by regulating IRAK1 expression in THP‑1 cells". Experimental and Therapeutic Medicine 18.4 (2019): 3078-3084.
Chicago
Zhou, C., Zhao, L., Wang, K., Qi, Q., Wang, M., Yang, L., Sun, P., Mu, H."MicroRNA‑146a inhibits NF‑κB activation and pro‑inflammatory cytokine production by regulating IRAK1 expression in THP‑1 cells". Experimental and Therapeutic Medicine 18, no. 4 (2019): 3078-3084. https://doi.org/10.3892/etm.2019.7881