Open Access

Gambogic acid suppresses colon cancer cell activity in vitro

  • Authors:
    • Zailong Zhou
    • Jian Ma
  • View Affiliations

  • Published online on: August 16, 2019     https://doi.org/10.3892/etm.2019.7912
  • Pages: 2917-2923
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to elucidate the underlying mechanism of antitumor activity of gambogic acid (GA) in colon cancer. Human colon cancer SW620 cells were divided into five treatment groups, including no‑treatment control (NC), low dose GA (10 µg/ml), medium dose GA (50 µg/ml), high dose GA (100 µg/ml) and 5‑fluorouracil (10 µg/ml). Differences in cell proliferation, apoptosis and cell cycle, invasion, and migration were measured between groups using MTT, flow cytometry, transwell and wound‑healing assays, respectively. Western blotting was used to analyze relative protein expression levels of phosphoinositide 3‑kinase (PI3K), protein kinase B (AKT), P21, and matrix metalloprotease (MMP)‑2 and ‑9 between groups. Compared with the NC group, GA (low, middle and high) inhibited SW620 cell proliferation, invasion and migration (all P<0.05). Furthermore, there were significant differences in proliferation, invasion and migration between groups administered with different doses of GA (all P<0.05). Compared with the NC group, the expression levels of PI3K, AKT, phosphorylated‑AKT, P21 and MMP‑2 and ‑9 were significantly altered in a dose dependent manner following treatment with GA (all P<0.05). The results of the current study indicated that GA suppressed proliferation and dispersion of human colon cancer cells in a dose‑dependent manner, possibly through a PI3K/AKT/P21/MMP‑2/9‑dependent pathway.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Zhou, Z., & Zhou, Z. (2019). Gambogic acid suppresses colon cancer cell activity in vitro. Experimental and Therapeutic Medicine, 18, 2917-2923. https://doi.org/10.3892/etm.2019.7912
MLA
Zhou, Z., Ma, J."Gambogic acid suppresses colon cancer cell activity in vitro". Experimental and Therapeutic Medicine 18.4 (2019): 2917-2923.
Chicago
Zhou, Z., Ma, J."Gambogic acid suppresses colon cancer cell activity in vitro". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2917-2923. https://doi.org/10.3892/etm.2019.7912