Open Access

Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells

  • Authors:
    • Hyunjin Lee
    • Sae Kyung Min
    • Youngmin Song
    • Yun‑Hee Park
    • Jun‑Beom Park
  • View Affiliations

  • Published online on: August 16, 2019     https://doi.org/10.3892/etm.2019.7904
  • Pages: 2867-2876
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was performed to evaluate the effects of short‑term application of bone morphogenetic protein‑7 (BMP‑7) on human gingiva‑derived mesenchymal stem cells with next‑generation sequencing. Human gingiva‑derived stem cells were treated with a final concentration of 100 ng/ml BMP‑7 and the same concentration of a vehicle control. mRNA sequencing and data analysis were performed along using gene ontology and pathway analysis. RT‑qPCR of mRNA of collagen I, Sp7, IBSP and western blot analysis of collagen I, osterix and bone sialoprotein was also performed. A total of 25,737 mRNAs were identified to be differentially expressed. Regarding osteoblast differentiation, 14 mRNAs were upregulated and 10 were downregulated when the results of the BMP‑7 at 3 h were compared with the control at 3 h. The expression of collagen I was increased following the application of BMP‑7 at 3 h, and this increase was also observed following western blot analysis. The effects of BMP‑7 on stem cells were evaluated with mRNA sequencing, and the expression was validated with RT‑qPCR and western blot analysis. The short‑term application of BMP‑7 produced an increased expression of collagen I, which was associated with target genes selected for osteoblast differentiation. This study may provide novel insights into the role of BMP‑7 using mRNA sequencing.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Lee, H., Min, S.K., Song, Y., Park, Y., & Park, J. (2019). Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells. Experimental and Therapeutic Medicine, 18, 2867-2876. https://doi.org/10.3892/etm.2019.7904
MLA
Lee, H., Min, S. K., Song, Y., Park, Y., Park, J."Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells". Experimental and Therapeutic Medicine 18.4 (2019): 2867-2876.
Chicago
Lee, H., Min, S. K., Song, Y., Park, Y., Park, J."Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2867-2876. https://doi.org/10.3892/etm.2019.7904