Open Access

Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts

  • Authors:
    • Monica Benvenuto
    • Rosanna Mattera
    • Martino Tony Miele
    • Maria Gabriella Giganti
    • Ilaria Tresoldi
    • Loredana Albonici
    • Vittorio Manzari
    • Andrea Modesti
    • Laura Masuelli
    • Roberto Bei
  • View Affiliations

  • Published online on: August 8, 2019     https://doi.org/10.3892/etm.2019.7872
  • Pages: 2639-2647
  • Copyright: © Benvenuto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The extracellular matrix (ECM) creates a tissue microenvironment able to regulate cellular signaling. The loss of ECM plasticity is associated with several pathologies, especially those involving chronic inflammation, therefore, the ECM represents a potential therapeutic target for certain conditions. The present study investigated the effects of a natural multi‑component compound formulation, Galium‑Heel®, on the growth, morphology and ECM production of human dermal fibroblasts (HDF). The effects of the formulation on HDF growth and morphology were assessed by sulforhodamine B assay, trypan blue exclusion staining, FACS and ultrastructural analyses. The effect of the compound on reactive oxygen species production by HDF was performed by dichlorofluorescin diacetate assay. The expression of ECM components, matrix metalloproteinases (MMPs) and signaling molecules was analyzed by western blot analysis. The present results demonstrated that Galium‑Heel® did not significantly affect HDF growth, survival, cell cycle or morphology indicating the biocompatibility of the formulation. The formulation demonstrated antioxidant activity. Galium‑Heel® was able to modulate ECM by regulating collagens (type I and III) and MMPs‑3 and ‑7 expression. In addition, the formulation was able to regulate molecules involved in TGF‑β signalling, including mitogen activated kinase‑like protein, GLI family zinc finger 2 and pro‑survival proteins such as AKT. The present results demonstrating the effects of a natural multi‑component compound on ECM composition, highlighted the possibility of pharmacologically modulating ECM molecules. The recovery and the maintenance of ECM homeostasis might be considered as a potential therapeutic goal to ameliorate pathological conditions.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Benvenuto, M., Mattera, R., Miele, M.T., Giganti, M.G., Tresoldi, I., Albonici, L. ... Bei, R. (2019). Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts. Experimental and Therapeutic Medicine, 18, 2639-2647. https://doi.org/10.3892/etm.2019.7872
MLA
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18.4 (2019): 2639-2647.
Chicago
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2639-2647. https://doi.org/10.3892/etm.2019.7872