Open Access

miR‑183 inhibits microglia activation and expression of inflammatory factors in rats with cerebral ischemia reperfusion via NF‑κB signaling pathway

  • Authors:
    • Bo Xiang
    • Ping Zhong
    • Lei Fang
    • Xianxian Wu
    • Yuqiang Song
    • Haicheng Yuan
  • View Affiliations

  • Published online on: July 30, 2019     https://doi.org/10.3892/etm.2019.7827
  • Pages: 2540-2546
  • Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemic stroke represents 87% of all strokes, and is the third leading cause of disability and mortality worldwide. The cause of ischemic stroke is the obstruction of blood flow through the artery that supplies oxygen‑rich blood to the brain, with ischemia‑reperfusion injury as its major cause. microRNAs (miRNA) are small non‑coding RNAs, which serve important roles in the regulation of gene expression at the post‑transcription level. The aim of the present study was to investigate the effect of miRNA‑183 (miR‑183) on microglia activation in rats with cerebral ischemia‑reperfusion injury. To this end, a rat cerebral ischemia‑reperfusion injury model was established. The results indicated that miR‑183 expression was decreased by cerebral ischemia‑reperfusion. In addition, treatment using miR‑183 agomir significantly reduced the neurological function scores, percentage of cerebral infarction volume, and ionized calcium‑binding adapter molecule‑1 (IBA‑1)‑positive cells in the CA1 area of the hippocampus in rats subjected to cerebral ischemia‑reperfusion injury, implicating a neuroprotective role for miR‑183. MiR‑183 agomir treatment also decreased the expression of pro‑inflammatory‑associated proteins interleukin (IL)‑1β, IL‑6 and tumor necrosis factor (TNF)‑α. Finally, the expression of the nuclear factor (NF)‑κB p65 and IκBα was decreased and increased by miR‑183 agomir treatment, respectively, indicating inhibition of the NF‑κB signaling pathway. These observations suggest that miR‑183 regulates the activation of microglia in rats with cerebral ischemia‑reperfusion injury by inhibiting the NF‑κB signaling pathway.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Xiang, B., Zhong, P., Fang, L., Wu, X., Song, Y., & Yuan, H. (2019). miR‑183 inhibits microglia activation and expression of inflammatory factors in rats with cerebral ischemia reperfusion via NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 18, 2540-2546. https://doi.org/10.3892/etm.2019.7827
MLA
Xiang, B., Zhong, P., Fang, L., Wu, X., Song, Y., Yuan, H."miR‑183 inhibits microglia activation and expression of inflammatory factors in rats with cerebral ischemia reperfusion via NF‑κB signaling pathway". Experimental and Therapeutic Medicine 18.4 (2019): 2540-2546.
Chicago
Xiang, B., Zhong, P., Fang, L., Wu, X., Song, Y., Yuan, H."miR‑183 inhibits microglia activation and expression of inflammatory factors in rats with cerebral ischemia reperfusion via NF‑κB signaling pathway". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2540-2546. https://doi.org/10.3892/etm.2019.7827