Open Access

Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE‑/‑ mice

  • Authors:
    • Hui Cao
    • Qingling Jia
    • Dingzhu Shen
    • Li Yan
    • Chuan Chen
    • Sanli Xing
  • View Affiliations

  • Published online on: August 5, 2019     https://doi.org/10.3892/etm.2019.7851
  • Pages: 2451-2458
  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study examined the involvement of autophagy as a mechanism in the protective effect of quercetin (QUE) on atherosclerosis (AS) in ApoE‑/‑ mice. An AS model was established by feeding ApoE‑/‑ mice a high‑fat diet (HFD). Mice were divided into four experimental groups: The model, QUE, 3‑methyladenine (3‑MA) and QUE + 3‑MA groups. Additionally, age‑matched wild‑type C57BL/6 mice were used as a Control group. Autophagosomes in the aorta were examined using a transmission electron microscope. Aorta pathology, serum lipid accumulation and collagen deposition were determined by hematoxylin and eosin, Oil Red O and Masson staining, respectively. The levels of cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β) and interleukin‑18 (IL‑18) were measured using ELISA assays. Protein levels of mTOR, microtubule associated protein 1 light chain 3a (LC3), P53 and cyclin dependent kinase inhibitor 1A (P21) in the aorta were analyzed using western blotting. ApoE‑/‑ mice which were fed HFD exhibited substantial AS pathology, no autophagosomes, higher levels of TNF‑α, IL‑1β, IL‑18 and mTOR and lower ratios of LC3 II/I. All these alterations were ameliorated and aggravated by QUE and 3‑MA treatment, respectively. The inhibition of AS by QUE may be associated with the enhancement of autophagy and upregulation of P21 and P53 expression.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Cao, H., Jia, Q., Shen, D., Yan, L., Chen, C., & Xing, S. (2019). Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE‑/‑ mice. Experimental and Therapeutic Medicine, 18, 2451-2458. https://doi.org/10.3892/etm.2019.7851
MLA
Cao, H., Jia, Q., Shen, D., Yan, L., Chen, C., Xing, S."Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE‑/‑ mice". Experimental and Therapeutic Medicine 18.4 (2019): 2451-2458.
Chicago
Cao, H., Jia, Q., Shen, D., Yan, L., Chen, C., Xing, S."Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE‑/‑ mice". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2451-2458. https://doi.org/10.3892/etm.2019.7851