Open Access

Elimination of HER3‑expressing breast cancer cells using aptamer‑siRNA chimeras

  • Authors:
    • Inga Nachreiner
    • Ahmad Fawzi Hussain
    • Ulrich Wullner
    • Nikolaus Machuy
    • Thomas F. Meyer
    • Rainer Fischer
    • Stefan Gattenlöhner
    • Ivo Meinhold‑Heerlein
    • Stefan Barth
    • Mehmet Kemal Tur
  • View Affiliations

  • Published online on: July 9, 2019     https://doi.org/10.3892/etm.2019.7753
  • Pages: 2401-2412
  • Copyright: © Nachreiner et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Breast cancer is the most common cancer in women worldwide. Despite recent developments in breast cancer detection and treatment, 1.38 million women each year are still affected. Breast cancer heterogeneity at the population and single‑cell level, complexity and developing different metastases are setting several challenges to develop efficient breast cancer therapies. RNA interference (RNAi) represents an opportunity to silence gene expression and inhibit specific pathways in cancer cells. In order to reap the full advantages of RNAi‑based therapy, different pathways that sustain cancer cells growth have been targeted using specific siRNAs. The present study investigated the ability of a set of cytotoxic siRNAs to inhibit growth of breast cancer cells. These siRNAs are targeting eukaryotic elongation factor 2 (EEF2), polo‑like kinase 1 (PLK1), G protein‑coupled receptor kinase 4 (GRK4) and sphingosine kinase interacting protein (SKIP5). To facilitate their targeted delivery, the human epidermal growth factor receptor‑3 (HER3)‑specific aptamer A30 was used. The in vitro results described in this work indicate that combining the highly specific HER3 aptamer with cytotoxic siRNAs targeting (EEF2, PLK1, GRK4 and SKIP5) can inhibit its activity and ultimately suppress proliferation of HER3 positive breast cancer cells.

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October 2019
Volume 18 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Nachreiner, I., Hussain, A.F., Wullner, U., Machuy, N., Meyer, T.F., Fischer, R. ... Tur, M.K. (2019). Elimination of HER3‑expressing breast cancer cells using aptamer‑siRNA chimeras. Experimental and Therapeutic Medicine, 18, 2401-2412. https://doi.org/10.3892/etm.2019.7753
MLA
Nachreiner, I., Hussain, A. F., Wullner, U., Machuy, N., Meyer, T. F., Fischer, R., Gattenlöhner, S., Meinhold‑Heerlein, I., Barth, S., Tur, M. K."Elimination of HER3‑expressing breast cancer cells using aptamer‑siRNA chimeras". Experimental and Therapeutic Medicine 18.4 (2019): 2401-2412.
Chicago
Nachreiner, I., Hussain, A. F., Wullner, U., Machuy, N., Meyer, T. F., Fischer, R., Gattenlöhner, S., Meinhold‑Heerlein, I., Barth, S., Tur, M. K."Elimination of HER3‑expressing breast cancer cells using aptamer‑siRNA chimeras". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2401-2412. https://doi.org/10.3892/etm.2019.7753