SIRT1 is dispensable for maturation of hematopoietic stem cell in the bone marrow niche

  • Authors:
    • Su Min Park
    • Jayoung Kim
    • Chae Mi Hong
    • Dong Hoon Shin
    • Jee Yeon Kim
    • Do Youn Park
    • Dong Hyun Sohn
    • Yun Hak Kim
    • Sang‑Mo Kwon
    • Jae Ho Kim
    • Sun Sik Bae
    • Koanhoi Kim
    • Chi Dae Kim
    • Chi‑Dug Kang
    • Dongjun Lee
  • View Affiliations

  • Published online on: July 25, 2019     https://doi.org/10.3892/etm.2019.7813
  • Pages: 2341-2345
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Abstract

Sirtuin 1 (SIRT1) is a histone deacetylase implicated in stem cell homeostasis. Conditional Sirt1 deletion in the hematopoietic stem and progenitor system promotes hematopoietic stem and progenitor cell (HSPC) expansion under stress conditions. In addition, SIRT1 activators modulate the capacity and HSPC numbers in the bone marrow (BM). To investigate the role of SIRT1 in the BM niche, a conditional Sirt1 deletion in the BM niche was generated in a mouse model for the present study. Multicolor flow cytometric analyses were performed to determine HSC cell populations. Using 5‑fluorouracil‑induced proliferative stress, a survival curve was produced. In the present study, Sirt1 deletion in the BM niche demonstrated that the production of mature blood cells, lineage distribution within hematopoietic organs and frequencies of HSPC populations were comparable to those of controls. Additionally, Sirt1 deletion in the BM niche did not perturb HSC maturation under stress induced by transplantation. Therefore, these observations suggest that SIRT1 serves a dispensable role in HSC maturation in the BM niche.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Park, S.M., Kim, J., Hong, C.M., Shin, D.H., Kim, J.Y., Park, D.Y. ... Lee, D. (2019). SIRT1 is dispensable for maturation of hematopoietic stem cell in the bone marrow niche. Experimental and Therapeutic Medicine, 18, 2341-2345. https://doi.org/10.3892/etm.2019.7813
MLA
Park, S. M., Kim, J., Hong, C. M., Shin, D. H., Kim, J. Y., Park, D. Y., Sohn, D. H., Kim, Y. H., Kwon, S., Kim, J. H., Bae, S. S., Kim, K., Kim, C. D., Kang, C., Lee, D."SIRT1 is dispensable for maturation of hematopoietic stem cell in the bone marrow niche". Experimental and Therapeutic Medicine 18.3 (2019): 2341-2345.
Chicago
Park, S. M., Kim, J., Hong, C. M., Shin, D. H., Kim, J. Y., Park, D. Y., Sohn, D. H., Kim, Y. H., Kwon, S., Kim, J. H., Bae, S. S., Kim, K., Kim, C. D., Kang, C., Lee, D."SIRT1 is dispensable for maturation of hematopoietic stem cell in the bone marrow niche". Experimental and Therapeutic Medicine 18, no. 3 (2019): 2341-2345. https://doi.org/10.3892/etm.2019.7813