Effect of sepsis on the action potential and cardiac serotonin response in rats

  • Authors:
    • Zheng-Jiang Liu
    • Hua Liu
    • Changdong Wu
    • Kedong Xue
  • View Affiliations

  • Published online on: July 25, 2019     https://doi.org/10.3892/etm.2019.7810
  • Pages: 2207-2212
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Abstract

The current study aimed to investigate the effect of sepsis on rat serotonin (5‑HT) responses and cardiac action potentials. A total of 20 rats were randomly divided into a sepsis and control group (each, n=10). Rat hearts were harvested and perfused using the Langendorff method 18‑h after the induction of sepsis, which was assessed using cecal puncture. Cardiac action potential was subsequently measured using a multichannel electrophysiology instrument. Immunohistochemistry and quantitative analysis were performed to identify the effect of sepsis on myocardial 5‑HT expression. The results revealed that mitochondrial changes were present in septic rat hearts. Heart rate (361.10±12.29 bpm vs. 348.60±12.38 bpm; P<0.05) was significantly higher, atrial action potential duration (106.40±2.95 ms vs. 86.60±4.12 ms; P<0.01) was significantly longer and the area (0.62±0.06 µm2 vs. 0.39±0.05 µm2; P<0.05) and number (0.92±0.02/field vs. 0.46±0.01/field; P<0.01) of myocardial cells were significantly higher in the septic compared with the control group. These results demonstrated that 5‑HT prolongs the atrial action potential, increases heart rate and aggravates myocardial injury, indicating that it may therefore be one of the factors that leads to cardiac dysfunction in sepsis.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Liu, Z., Liu, H., Wu, C., & Xue, K. (2019). Effect of sepsis on the action potential and cardiac serotonin response in rats. Experimental and Therapeutic Medicine, 18, 2207-2212. https://doi.org/10.3892/etm.2019.7810
MLA
Liu, Z., Liu, H., Wu, C., Xue, K."Effect of sepsis on the action potential and cardiac serotonin response in rats". Experimental and Therapeutic Medicine 18.3 (2019): 2207-2212.
Chicago
Liu, Z., Liu, H., Wu, C., Xue, K."Effect of sepsis on the action potential and cardiac serotonin response in rats". Experimental and Therapeutic Medicine 18, no. 3 (2019): 2207-2212. https://doi.org/10.3892/etm.2019.7810