Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a

  • Authors:
    • Hua‑Jun Wu
    • Guan‑Min Tang
    • Ping‑Yang Shao
    • Hong‑Xing Zou
    • Wei‑Feng Shen
    • Ming‑De Huang
    • Hang‑Hai Pan
    • Chang‑Lin Zhai
    • Gang Qian
  • View Affiliations

  • Published online on: July 17, 2019     https://doi.org/10.3892/etm.2019.7788
  • Pages: 2199-2206
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Abstract

In the present study, a hypoxia/reoxygenation (H/R) model of cardiomyocytes was established to investigate the effects of long non‑coding RNA (LncRNA) Nuclear Enriched Abundant Transcript 1 (NEAT1) and microRNA (miR)‑520a on H/R‑induced cardiomyocyte apoptosis. Flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling staining were used to evaluate cell apoptosis. Luciferase activity assay was used to investigate whether miR‑520a targets NEAT1. Results revealed that NEAT1 was significantly upregulated and miR‑520a was downregulated in the ischemia/reperfusion myocardium and the cardiomyocytes that received H/R treatment. Further study demonstrated that knockdown of NEAT1 and overexpression of miR‑520a serves a protective role against H/R‑induced cardiomyocyte apoptosis. miR‑520a directly targets NEAT1 and its expression level is negatively correlated with that of NEAT1. The findings suggested that NEAT1 and miR‑520a may protect cardiomyocytes from apoptosis through regulating apoptotic proteins B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein, and altering cleaved caspase3 expression levels.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Wu, H., Tang, G., Shao, P., Zou, H., Shen, W., Huang, M. ... Qian, G. (2019). Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a. Experimental and Therapeutic Medicine, 18, 2199-2206. https://doi.org/10.3892/etm.2019.7788
MLA
Wu, H., Tang, G., Shao, P., Zou, H., Shen, W., Huang, M., Pan, H., Zhai, C., Qian, G."Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a". Experimental and Therapeutic Medicine 18.3 (2019): 2199-2206.
Chicago
Wu, H., Tang, G., Shao, P., Zou, H., Shen, W., Huang, M., Pan, H., Zhai, C., Qian, G."Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a". Experimental and Therapeutic Medicine 18, no. 3 (2019): 2199-2206. https://doi.org/10.3892/etm.2019.7788