Open Access

COL6A1 knockdown suppresses cell proliferation and migration in human aortic vascular smooth muscle cells

  • Authors:
    • Zongxiang Chen
    • Qingjian Wu
    • Chengjun Yan
    • Juan Du
  • View Affiliations

  • Published online on: July 19, 2019     https://doi.org/10.3892/etm.2019.7798
  • Pages: 1977-1984
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Vascular smooth muscle cell (VSMC) migration is an important pathophysiological signature of neointimal hyperplasia. The aim of the present study was to investigate the effects of collagen type VI α1 chain (COL6A1) on VSMC migration. COL6A1 expression was silenced in platelet‑derived growth factor (PDGF‑BB)‑stimulated VSMCs. Cell counting kit‑8, wound healing and Transwell assays were used to measure cell viability, migration and invasion, respectively. Reverse transcription‑quantitative PCR and western blot analysis were performed to analyze the expression of factors associated with metastasis. COL6A1 silencing attenuated PDGF‑BB‑induced increases in cell viability and invasive abilities of VSMCs, in addition to partially reversing the increased expression of fibronectin (FN), matrix metalloproteinase (MMP)‑2 and MMP‑9 induced by PDGF‑BB stimulation. The silencing of COL6A also overturned PDGF‑BB‑induced reduction in tissue inhibitor of metalloproteinase 2 expression in VSMCs. PDGF‑BB activated the AKT/mTOR pathway, which was also inhibited by COL6A1 knockdown. Taken together, these findings suggest that COL6A1 silencing inhibited VSMC viability and migration by inhibiting AKT/mTOR activation.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Chen, Z., Wu, Q., Yan, C., & Du, J. (2019). COL6A1 knockdown suppresses cell proliferation and migration in human aortic vascular smooth muscle cells. Experimental and Therapeutic Medicine, 18, 1977-1984. https://doi.org/10.3892/etm.2019.7798
MLA
Chen, Z., Wu, Q., Yan, C., Du, J."COL6A1 knockdown suppresses cell proliferation and migration in human aortic vascular smooth muscle cells". Experimental and Therapeutic Medicine 18.3 (2019): 1977-1984.
Chicago
Chen, Z., Wu, Q., Yan, C., Du, J."COL6A1 knockdown suppresses cell proliferation and migration in human aortic vascular smooth muscle cells". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1977-1984. https://doi.org/10.3892/etm.2019.7798