Isopsoralen ameliorates H2O2‑induced damage in osteoblasts via activating the Wnt/β‑catenin pathway

  • Authors:
    • Yu‑Peng Li
    • Bin Wu
    • Jie Liang
    • Fei Li
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/etm.2019.7741
  • Pages: 1899-1906
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Abstract

Osteoporosis is a disease with a worldwide prevalence that involves a severe loss of bone mineral density and decreased microarchitecture, which increases the risk of bone fracture. The present study evaluated the effects of isopsoralen on osteoblastic OB‑6 cells following hydrogen peroxide (H2O2)‑induced damage and investigated the molecular mechanisms involved in this process. For in vitro experiments, OB‑6 osteoblasts were treated with H2O2 or H2O2 + isopsoralen then the cell viability, apoptosis, reactive oxygen species (ROS) production and calcium accumulation were determined. Results demonstrated that treatment with H2O2 reduced cell viability, runt‑related transcription factor 2 (RUNX2) and osteocalcin (OCN) expression levels, and calcium deposition, whilst markedly increasing cell apoptosis and ROS production. However, isopsoralen (1 µM) provided significant protection against H2O2‑induced alterations in osteoblasts. In addition, isopsoralen effectively upregulated protein expression of tankyrase and β‑catenin which are the main transductors of the Wnt/β‑catenin pathway. Of note, the protective effects of isopsoralen against H2O2‑induced damage were attenuated in OB‑6 cells treated with tankyrase inhibitor XAV‑939. In conclusion, the present findings provided evidence that isopsoralen attenuated oxidative stress‑induced injury in osteoblasts via the Wnt/β‑catenin signaling pathway.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Li, Y., Wu, B., Liang, J., & Li, F. (2019). Isopsoralen ameliorates H2O2‑induced damage in osteoblasts via activating the Wnt/β‑catenin pathway. Experimental and Therapeutic Medicine, 18, 1899-1906. https://doi.org/10.3892/etm.2019.7741
MLA
Li, Y., Wu, B., Liang, J., Li, F."Isopsoralen ameliorates H2O2‑induced damage in osteoblasts via activating the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 18.3 (2019): 1899-1906.
Chicago
Li, Y., Wu, B., Liang, J., Li, F."Isopsoralen ameliorates H2O2‑induced damage in osteoblasts via activating the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1899-1906. https://doi.org/10.3892/etm.2019.7741